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*All samples concentrated using centrifuge concentrators. +. +. +. +. +. +. -. -. -. -. +. +. -. +. +. -. 48hrs. 24hrs. 48hrs. 48hrs. 24hrs. 48hrs. 48hrs. 48hrs. 196. 102. 57. 41. 27. 20. 15. 6. Wild-Type Media. FOP Media. TNF- α. IFN- γ. Pure TGF- ß. Time.

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  1. *All samples concentrated using centrifuge concentrators + + + + + + - - - - + + - + + - 48hrs 24hrs 48hrs 48hrs 24hrs 48hrs 48hrs 48hrs 196 102 57 41 27 20 15 6 Wild-Type Media FOP Media TNF-α IFN-γ Pure TGF-ß Time ~110KD ~50-55KD TGF-ß like peptide Seems as though 48hr stimulation with TNF-alpha is associated with the greatest reduction in TGF-B secretion, more so than IFNy + TNF-alpha

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