Exploring Calcium Dynamics in Ventricular Myocytes Using Cameleon YC2.1 and Caffeine Stimulation

Feb 17, 2026

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This study investigates the calcium dynamics in ventricular myocytes post-infection with an adenoviral construct of the calcium-sensitive probe Cameleon YC2.1. Fluorescent imaging reveals time-dependent changes in cyan and yellow fluorescence signals after administering 10 mM caffeine. Observations show a decreased FRET efficiency, indicating reduced intracellular calcium levels detected by the probe. These findings suggest the probe's localization within the sarcoplasmic reticulum (SR) and provide insights into cellular calcium signaling mechanisms in response to caffeine applications.

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Exploring Calcium Dynamics in Ventricular Myocytes Using Cameleon YC2.1 and Caffeine Stimulation

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B A Cyan caffeine 200 180 Intensity, a.u. 160 Time, s 140 0 50 100 150 Yellow 0 -5 DR/R0*100, % -10 -15 20 mm Supplemental Figure. A, XY images of ventricular myocyte 48 ours after infection with adenoviral construct of cameleon YC2.1 flanked with ER-retention sequence (KDEL). B, Time-dependent changes of Cyan and Yellow fluorescence signals upon application of 10 mM caffeine (upper panel). Decreased FRET efficiency (lower panel) in response to caffeine reflects decreased [Ca2+] sensed by the probe. This type of response consistent with probe localization in the SR compartment.