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Relative Quantities

P. acnes -primed. 2  g LPS. 6. Pai1. tpa. 60. 5. 50. 4. 40. 3. 30. 2. 20. 1. 10. 0. 0. 0. 0. 1.5. 1.5. 4. 4. 8. 8. * p <0.05 , ** p <0.01. *. **. Relative Quantities. Post LPS (h). Kawa, et al. Supplementary Fig. 1. 8. 1.0. 0.8. 6. 0.6. 4. 0.4. 2. 0.2. 0.

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Relative Quantities

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  1. P. acnes-primed 2 g LPS 6 Pai1 tpa 60 5 50 4 40 3 30 2 20 1 10 0 0 0 0 1.5 1.5 4 4 8 8 *p<0.05, **p<0.01 * ** Relative Quantities Post LPS (h) Kawa, et al. Supplementary Fig. 1

  2. 8 1.0 0.8 6 0.6 4 0.4 2 0.2 0 0 Naive Naive P. acnes P. acnes Naive Naive P. acnes P. acnes WT WT Il12p40-/- Il12p40-/- Splenocytes *** p<0.001 IL-4 IFN- (ng/ml) ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND 0 1 10 100 Heat-killed P. acnes (g/ml) Kawa, et al. Supplementary Fig. 2

  3. Pretreatment with Med rIL-12 Med rIL-12 Med rIL-12 rIFN- rIFN- rIFN- WT Il12p40-/- Ifn-/- 6 TNF- *** *** *** ** ** ** 5 ns ns ns ns ns ns 4 3 2 1 ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND 0 14 IL-6 12 10 8 6 4 2 0 0 10-1100101102103 LPS (ng/ml) BMM **p<0.01, *** p<0.001 (ng/ml) Kawa, et al. Supplementary Fig. 3

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