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Advisor : Cheng-Hsin Chuang Advisee :Wan-Ting Su Department of Mechanical Engineering PowerPoint Presentation
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Advisor : Cheng-Hsin Chuang Advisee :Wan-Ting Su Department of Mechanical Engineering

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Advisor : Cheng-Hsin Chuang Advisee :Wan-Ting Su Department of Mechanical Engineering

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  1. Advisor : Cheng-Hsin Chuang Advisee :Wan-Ting Su Department of Mechanical Engineering & Institute of Nanotechnology, Southern Taiwan University, Tainan, TAIWAN Date ﹕2012/04/06 Innovate Food Science and Emerging Technologies 12 (2011) 617-622 A microwire sensor for rapid detection of Escherichia coli K-12 in fresh produce Sungkyun Kim ,Lin Lu, Jae-Hyun Chung ,Kyonghoon Lee ,Yong Li ,Soojin Jun PaperSurvey

  2. Outline • PaperSurvey • Sensor Design • Results and discussion • Experimental Result • Future work

  3. Sensor Design Fig .1. (a) Microwire sensing unit (b) Power supply and measurement equipments

  4. Results and Discussion Fig2.Regression curve for enumeration of E.coli K-12cells Fig3.Stepwise produres for detection of E.coli K-12 DEP force and electroosmotic flow Capillary

  5. Simulation Result Fig 4. Sequential simulation of electric field strength

  6. Experiment Results Fig. 5. FESEM image of E.coli K-12 cells captured on the microwire. (a)Without and (b) with AC field ﹒

  7. Experiment Results Fig. 6. Optimization of the working frequency for detection of E.coli K-12 at 106 CFU/ml Fig .7. Fluorescence intensity of E.coli K-12 in PBS buffer when applied with 20VPP and 3MHz

  8. Experiment Results Fig. 8. Selective capture of bactrial cells from E.coli K-12 internalized spinach solution: (a) FI values of E.coli K-12 in spinach and buffer solutions at different concentrations (b) FSEM image of the microwire sensor. Many spinach particles are captured on the wire surface

  9. Outline • PaperSurvey • Sensor Design • Results and discussion • Experimental Result • Future work

  10. Experimental Experimental Method

  11. Experimental Results Applied Time : 0 min Applied Time : 2 min Flow Rate : 0.1 ml/hr Al2O3 Concentration:2 wt% BSA Concentration:1 μM Applied Frequency:10 kHz Apply Voltage:10 Vpp Applied Time : 16 min Applied Time : 30 min

  12. Outline • PaperSurvey • Sensor Design • Results and discussion • Experimental Result • Future work

  13. Future work • PaperSurvey • Experiment by Different Parameter • Experimental Sample Using Bladder Cancer Cell • SEM Training

  14. Thank you for your attention!!