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Impact of Nitric Oxide Species on Nitrate and Nitrite Reduction in Engineered Microbial Systems

This study investigates the effects of various nitrogen compounds, including nitrate (NO3-), nitrite (NO2-), nitric oxide (NO), and nitrous oxide (N2O), on microbial activity under controlled laboratory conditions. Utilizing engineered strains with modified narG and nirS genes, we examine how these bacteria perform in a nutrient-rich LB medium at pH 7.8 with 15 mM concentrations of NO3- and NO2-. The implications for bioremediation and nitrogen cycling are discussed, providing insights into microbial responses to different nitrogen oxides.

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Impact of Nitric Oxide Species on Nitrate and Nitrite Reduction in Engineered Microbial Systems

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  1. F H E G D C B A NAR NIR NOR NOS NO3- NO2- NO N2O N2 +O2 -O2 -O2 PAO1 * ΔnarG * J I ΔnirS LB + 15 mM NO3- LB (pH7.8) +15 mM NO2-

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