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Biosinteza proteinov

Biosinteza proteinov. Voet 3: poglavje 32 Stryer 5: poglavje 29 Krebs 3: poglavji 25 in 24. http://plato.stanford.edu/entries/information-biological/. s tandardni začetni kodon: AUG nestandardni: GUG, UUG, … E. coli : AUG – 83 % mRNA (Met) GUG – 14 % mRNA ( Val )

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Biosinteza proteinov

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  1. Biosinteza proteinov Voet 3: poglavje 32 Stryer 5: poglavje 29 Krebs 3: poglavji 25 in 24

  2. http://plato.stanford.edu/entries/information-biological/

  3. standardni začetni kodon: AUG nestandardni: GUG, UUG, … E. coli: AUG – 83 % mRNA (Met) GUG – 14 % mRNA (Val) UUG – 3 % mRNA (Leu) AUU CUG Tudi pri alternativnih start kodonih je najpogosteje 1. aminokislina Met!

  4. Odstopanja od “standardnega” genetskega koda pri mitohondrijih.

  5. Adapterskahipoteza: genetski kod berejo molekule, ki prepoznajo posamezne kodone, hkrati pa nosijo ustrezne aminokisline.

  6. Nukleotidno zaporedje in oznake krakov na primeru kvasovkine tRNAAla, prikazane v strukturi deteljice.

  7. UH2=metilinozindihidrouridin mG=metilgvanozin m2G=dimetilgvanozin T=ribotimidin I=inozin mI=metilinozin =psevdouridin

  8. p = (1-ε)n

  9. Nature Reviews Microbiology 8, 849-856 (Dec.2010)

  10. Nekatere bakterije, arheje, mitohondriji in kloroplasti nimajo GlnRS; pri njih se Glu-tRNA encimsko transamidira v Gln-tRNA.

  11. poli-Lys se ne obarja s TCA!!! (Ochoa, Watson)

  12. stabilni brezcelični sistem E. coli • mešanica 19 neradioaktivnih + 1 radioaktivno označene aminokisline ( 20 paralelk z vsakič drugo označeno AK) • dodatek (m)RNA • inkubiranje • obarjanje proteinov & spiranje precipitata • merjenje radioaktivnosti v oborini • pri poli-U so izmerili radioaktivnost le v paralelki z označenim Phe

  13. degeneriranost genetskega koda • ohlapne baze

  14. Adenozin-deaminaza (ADA): katabolizem purinov SCID (huda kombinirana imunska pomanjkljivost: posledica dedne okvare ADA http://themedicalbiochemistrypage.org/scid.html

  15. Lodish 4 Zorenje evkariontske rRNA

  16. snoRNP: mali nukleolarniribonukleoproteinski delec

  17. rRNA 16 S 1542 nt

  18. Nekateri proteini, ki sestavljajo ribosomsko podenoto 50 S.

  19. Trije možni bralni okviri na mRNA. Vsak bralni okvir pomeni drugačen polipeptid, ki lahko nastane na osnovi istega zaporedja nukleotidov.

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