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This study utilizes semi-quantitative RT-PCR to confirm microarray data involving 21 genes regulated by drought during critical developmental stages in rice: tillering, panicle elongation, and booting. The microarray results are displayed on the left, while RT-PCR outcomes are presented on the right. Key indicators like TLC, TLS, TRC, and TRS represent leaves and roots under control and stress conditions at the tillering stage, while PLC, PLS, PRC, and PRS correspond to the panicle elongation stage. BLC, BLS, BPC, and BPS reflect measurements at the booting stage.

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  1. -3 -2 -1 0 1 2 3 Additional file 5. Semi-quantitative RT-PCR confirmation of microarray data. The microarray data was shown on the left side, and RT-PCR results were shown on the right side. Twenty-one genes differentially regulated by drought at tillering stage, panicle elongation stage and booting stage. TLC and TLS, TRC and TRS indicate leave under control and under stress, root under control and stress at tillering stage respectively; PLC and PLS, PRC and PRS indicate leave under control and stress, root under control and stress at panicle elongation stage respectively; BLC and BLS, BPC and BPS indicate leave under control and stress, panicle under control and stress at booting stage respectively.

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