Peptides

1. References listed in Table 1S • Akoun, G.M. et al. (1985. Serum neurone-specific enolase, A marker for disease extent and response to therapy for small-cell lung cancer. Chest. 87, 39-43 • Eustace, B. K., and Jay, D.G. (2004) Extracellular roles for the molecular chaperone, hsp90. Cell Cycle, 3, 1098-1100 • Gurney, M. E., et al. (1986) Neuroleukin: a lymphokine product of lectin-stimulated T cells. Science. 234, 574-581 • Katz, A. B., and Taichman, L. B. (1999) A partial catalog of proteins secreted by epidermal keratinocytes in culture. J. Invest. Dermatol.112, 818-821 • Martin-Ventura, J. L., et al. (2004) Identification by a differential proteomic approach of heat shock protein 27 as a potential marker of atherosclerosis. Circulation. 110, 2216-2219 • Nodes, B. R., Shackelford, J. E., Lebherz, H. G. (1987) Synthesis and secretion of serum gelsolin by smooth muscle tissue. J. Biol. Chem. 262, 5422-5427 • Oremek, G. M., Teigelkamp, S., Kramer, W., Eigenbrodt, E., and Usadel, K.H. (1999) The pyruvate kinase isoenzyme tumor M2 (Tu M2-PK) as a tumor marker for renal carcinoma. Anticancer Res. 19(4A), 2599-2601 • Rokeach, L. A., et al. (1991) Characterization of the autoantigen calreticulin. J Immunol. 147, 3031-3039 • Rothhut, B., Russo-Marie, F., Wood, J., DiRosa, M., and Flower, R. J. (1983) Further characterization of the glucocorticoid-induced antiphospholipase protein "renocortin". Biochem Biophys Res Commun.117, 878-884 Table 1S. Results of mass spectrometry analyses # 1st Match Score/MP Reference Peptides * 1 Nodes et al. 1987 110 kDa 116/4 Gelsolin * 629/21 heat shock protein90 93 kDa Eustace and Jay, 2004 2 * pyruvate kinase M2 73 kDa 423/14 Oremek et al, 1999 3 * calreticulin Rokeach et al, 1991 4 70 kDa 235/10 * Autocrine motility factor (AMF, neuroleukin, GIP) 5 180/9 58 kDa Gurney et al, 1986 - 6 56 kDa 247/6 Fibronectin 1 isoform 3 52 kDa 7 unclear NA 50 kDa 8 NA - unclear * 45 kDa 9 Enolase 1 Akoun et al. 1985 724/35 10 278/12 43 kDa  cytoskeletal actin - 11 42 kDa NA unclear 12 40 kDa Rothhut et al. 1983 Annexin I (lipocortin I) 265/11 Fibronectin isoform 6 HSP70 Fragment - 13 35 kDa 271/15; 214/17 * 32 kDa 14-3-3 zeta Katz et al. 1999 14 435/34 * Katz et al. 1999 30 kDa 375/25 15 14-3-3 sigma (stratifin) * 29.5 kDa Hsp-27 Martin-Ventura et al. 1999 16 101/3 25 kDa 17 unclear NA 18 18 kDa unclear NA 19 16 kDa unclear NA *Previously reported as a secreted molecules; “unclear”, not high enough score to draw a conclusion; NA, not applicable; The list of references is given at the end of Materials and Methods