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Characterization of Mutant Strains ΔhpdA, ΔhpdB, and ΔhpdC in R20291

This study investigates the genetic and phenotypic properties of the mutant strains ΔhpdA, ΔhpdB, and ΔhpdC derived from the R20291 strain. The analysis includes the assessment of deletion mutants using primers hpdB-F/R and hpdCA-F, alongside EBS and RAM techniques. Size assessment through various loci provides insight into the genetic structure, with results indicating distinct molecular weights for each strain. The findings contribute to the understanding of gene function in pathogenicity and resistance in the R20291 strain.

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Characterization of Mutant Strains ΔhpdA, ΔhpdB, and ΔhpdC in R20291

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  1. A) 784a ΔhpdB ΔhpdA hpdB-F/R hpdCA-F/hpdA-R EBS/hpdB-R RAM-F/R EBS/hpdCA-F RAM-F/R M1 M2 W P - M1 M2 W P - M1 M2 W P - Size bp Size bp M W P - M W P - M W P - 2577 3065 1250 1250 850 1165 677 850 487 718 R20291 R20291 630erm 630erm 630erm R20291 254s 177s B) ΔhpdC hpdCA-F/hpdC-R EBS/hpdCA-F RAM-F/R M1 M2 W M1 M2 W P - M1 M2 W M1 M2 W P - M1 M2 W M1 M2 W P - Size bp 2130 1250 850 391 330 630erm R20291 630erm 630erm R20291 R20291 ΔhpdB ΔhpdC ΔhpdA ΔhpdC C) P W M1 M2 M3 P W M1 M2 M3 M4 bp bp 23,130 23,130 9,416 9,416 6,557 6,557 4,361 4,361 2,322 2,322 2,027 2,027 R20291 630Δerm

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