1 / 8

Restriction Enzymes

Restriction Enzymes. Micah Matthews and Hannah Perryman. What is the role of a restriction enzyme?. To cut DNA molecules at very precise sequences of 4 to 8 base pairs called recognition sites Named according to bacterial species from which they were first isolated

zelia
Télécharger la présentation

Restriction Enzymes

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Restriction Enzymes Micah Matthews and Hannah Perryman

  2. What is the role of a restriction enzyme? • To cut DNA molecules at very precise sequences of 4 to 8 base pairs called recognition sites • Named according to bacterial species from which they were first isolated • Allows genetic engineers to isolate, sequence, and manipulate individual genes derived from any type of organism

  3. Sticky End Restriction Enzymes • The restriction enzyme scans the DNA for a specific sequence of base pairs, known as the recognition site. • The cut results in a DNA fragment with two overhanging sticky ends, ends with exposed nucleotide bases.

  4. A Visual of Sticky End Restriction Enzymes http://www.dnalc.org/resources/animations/restriction.html

  5. Blunt End Restriction Enzymes • The restriction enzyme scans the DNA for a specific sequence of base pairs, known as the recognition site. • The cut results in a DNA fragment with two blunt ends, ends with NO exposed nucleotide bases.

  6. Visual of Blunt End Restriction Enzymes

  7. How is this used? • More than 400 restriction enzymes have been isolated from bacteria (see packet for examples). • In live bacteria, restriction enzymes defend the cell against invading bacteriophages

  8. Sources • http://www.mhhe.com/biosci/esp/2001_gbio/folder_structure/ge/m6/s1/assets/images/gem6s1_1.jpg • http://www.uic.edu/classes/phar/phar331/lecture5/image15.gif

More Related