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Protocol for ELISA Sample Submission-384 well : General Procedures

Protocol for ELISA Sample Submission-384 well : General Procedures Primary assays: x-40 & x-42 . (1-40, 1-42, & 1-total on special request). Linear range: 1pM (4pg/ml) - 110pM (500pg/ml). 50uL /well is needed for each assay. If both x-40 and x-42, submit 90uL.

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Protocol for ELISA Sample Submission-384 well : General Procedures

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  1. Protocol for ELISA Sample Submission-384 well: General Procedures • Primary assays: x-40 & x-42. (1-40, 1-42, & 1-total on special request). • Linear range: 1pM (4pg/ml) - 110pM (500pg/ml). • 50uL/well is needed for each assay. If both x-40 and x-42, submit 90uL. • 2G3 and 21F12 capture antibodies used for x-40 and x-42 assays, respectively; 266B antibody used for detection. • Submit samples on 96 well plate!! • Please DO NOT use tissue culture plates. • Please leave 1 row empty for peptide standard, and 1 column empty. • Always submit a submission sheet ahead of time. Even if you talk to Ting about preparing a submission, you should submit a sheet at that time. This is the ONLY way that Ting can plan ahead and prepare for assays. Submission sheets can be emailed to Ting (tyang3@partners.org) or dropped off at Ting’s desk (Harvard Institute of Medicine, Xia Lab, HIM 610).

  2. Protocol for ELISA Sample Submission-duplicate: sample layout • --For 1 assay submit 50ul/sample; for 2 assays(x-40 & x-42) submit 90ul/well • --Add samples to a 96 well plate, starting at B1 and moving across, as shown on left below. • --Fill all 11 wells in each row before starting another row. • --Fill up to 7 rows. Your sample layout (on 96 well plate): 1 2 3 4 5 6 7 8 9 10 11 12 A B C D E F G H

  3. Example of sample submission sheet 384 well duplicate

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