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Restriction Analysis of Plasmids pAMP and pKAN in Recombinant DNA Experiments

This report details the restriction digest analysis conducted on plasmids pAMP and pKAN across multiple laboratory sessions. Multiple gels demonstrate control and experimental lanes, including undigested and digested samples, as well as ligated DNA results. The work involved various groups, such as Hania, Nejla, Jayant, and Rashmi, and aims to visualize the digestion patterns of plasmids and recombinant DNA through electrophoresis. The findings provide insights into DNA manipulation techniques vital for molecular biology research.

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Restriction Analysis of Plasmids pAMP and pKAN in Recombinant DNA Experiments

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  1. Bio370 Wednesday Gels

  2. Hania, Nejla, Jayant, Rashmi • Gel from Lab 1: Restriction digest of plasmids pAMP and pKAN • Lanes from left to right: Marker, control pAMP, digested pAMP, digested pKAN, control pKAN • Gel from Lab 4b: Restriction analysis of purified recombinant DNA • Lanes from left to right: Marker, undigested M1, undigested M2, control pAMP, control pKAN, digested M1, digested M2, digested pAMP/pKAN, ligated DNA from lab 1

  3. Hania, Nejla, Jayant, Rashmi (Additional gel) Gel from Lab 4b: Restriction analysis of purified recombinant DNA ( From 2004 winter semestre) • Lanes from left to right: Marker, undigested M1, undigested M2, control pAMP, digested M1, digested M2, digested pAMP/pKAN, ligated DNA from lab 1

  4. Tiffany, Jade, Adrienne, Caroline • Gel from Lab 1: Restriction digest of plasmids pAMP and pKAN • Lanes from left to right: Marker, control pAMP, digested pAMP, digested pKAN, control pKAN • Gel from Lab 4b: Restriction analysis of purified recombinant DNA • Lanes from left to right: Marker, undigested M1, undigested M2, control pAMP, control pKAN, digested M1, digested M2, digested pAMP/pKAN, ligated DNA from lab 1

  5. Bianco, Jasneen, Alex, Amjad • Gel from Lab 1: Restriction digest of plasmids pAMP and pKAN • Lanes from left to right: Marker, control pAMP, digested pAMP, digested pKAN, control pKAN • Gel from Lab 4b: Restriction analysis of purified recombinant DNA • Lanes from left to right: Marker, undigested M1, undigested M2, control pAMP, control pKAN, digested M1, digested M2, digested pAMP/pKAN, ligated DNA from lab 1

  6. Bianco, Jasneen, Alex, Amjad (additional gel) Gel from Lab 4b: Restriction analysis of purified recombinant DNA ( From 2004 winter semestre) • Lanes from left to right: Marker, undigested M1, undigested M2, control pAMP, digested M1, digested M2, digested pAMP/pKAN, ligated DNA from lab 1

  7. Woo, Akash, Chintan, Ketan • Gel from Lab 1: Restriction digest of plasmids pAMP and pKAN • Lanes from left to right: Marker, control pAMP, digested pAMP, digested pKAN, control pKAN • Gel from Lab 4b: Restriction analysis of purified recombinant DNA • Lanes from left to right: Marker, undigested M1, undigested M2, control pAMP, control pKAN, digested M1, digested M2, digested pAMP/pKAN, ligated DNA from lab 1

  8. Mark, Robert, Caio • Gel from Lab 1: Restriction digest of plasmids pAMP and pKAN • Lanes from left to right: Marker, control pAMP, digested pAMP, digested pKAN, control pKAN • Gel from Lab 4b: Restriction analysis of purified recombinant DNA • Lanes from left to right: Ligated DNA from lab 1, marker, undigested M1, undigested M2, control pAMP, control pKAN, digested M1, digested M2, digested pAMP/pKAN

  9. Vanitha, Olivia, Avon, Misu • Gel from Lab 1: Restriction digest of plasmids pAMP and pKAN • Lanes from left to right: Marker, control pAMP, digested pAMP, digested pKAN, control pKAN • Gel from Lab 4b: Restriction analysis of purified recombinant DNA • Lanes from left to right: Marker, undigested M1, undigested M2, control pAMP, control pKAN, digested M1, digested M2, digested pAMP/pKAN, ligated DNA from lab 1

  10. Vanitha, Olivia, Avon, Misu (Additional gel) Gel from Lab 4b: Restriction analysis of purified recombinant DNA ( From 2004 winter semestre) • Lanes from left to right: Marker, undigested M1, undigested M2, control pAMP, digested M1, digested M2, digested pAMP/pKAN, ligated DNA from lab 1

  11. Mike’s Group (MANB) • Gel from Lab 1: Restriction digest of plasmids pAMP and pKAN • Lanes from left to right: Marker, control pAMP, digested pAMP, digested pKAN, control pKAN • Gel from Lab 4b: Restriction analysis of purified recombinant DNA • Lanes from left to right: Marker, undigested M1, undigested M2, control pAMP, control pKAN, digested M1, digested M2, digested pAMP/pKAN, ligated DNA from lab 1

  12. Stephanie’s Group (SSS) • Gel from Lab 1: Restriction digest of plasmids pAMP and pKAN • Lanes from left to right: Marker, control pAMP, digested pAMP, digested pKAN, control pKAN • Gel from Lab 4b: Restriction analysis of purified recombinant DNA • Lanes from left to right: Marker, undigested M1, undigested M2, control pAMP, control pKAN, digested M1, digested M2, digested pAMP/pKAN, ligated DNA from lab 1

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