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This study explores the use of pulsed laser-generated multiphoton excitation to fabricate micron-scale hydrogel matrices from biopolymer solutions, focusing on tuning their functional and mechanical properties. The ultimate goal is to create hydrogel microstructures suitable for neural tissue engineering applications, specifically in designing cell scaffolds. Methods involve dynamic mask multiphoton lithography and adjustments to Young's modulus using varying concentrations of BSA and GMHA gels, enhancing the hydrogels' capabilities for cellular microenvironments.
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Tuning the Functional and Mechanical Properties of Multiphoton Fabricated Hydrogels 20 µm Eric Spivey 24 May 2011
Biopolymer Solution Focused, pulsed laser Background: Using pulsed laser-generated multiphoton excitation to fabricate micron scale hydrogel matrices from biopolymer solutions. Biopolymer Hydrogel Matrix Microscope Objective Motivation: Use this method to fabricate cell scaffolds for neural tissue engineering applications. Goal:Fabricate hydrogel microstructures with tunable functional and mechanical properties.
Cellular Microenvironments 10 µm 25 µm
Tuning Young’s Modulus 5 µm 20 µm 10 µm
Tuning Young’s Modulus BSA and GMHA Gels PEG and GMHA Gels
Functionalizing Hydrogels 1 mW 2 mW 3 mW 4 mW 20 µm
Ongoing Research 20 µm
