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Amine-presenting electro-conducting scaffolds

This study explores Amino-Polypyrrole (APPy) as a novel electro-conducting scaffold for enhancing neuronal adhesion. Neurons typically exhibit poor adherence to standard Polypyrrole (PPy) films, necessitating external treatments with various ECM molecules or polycationic polymers. APPy, characterized by its positive charge, offers the potential for increased cell adhesion and a permanent display of this charge on the substrate. This makes APPy an excellent candidate for interfacing materials between neurons and electrodes in neural probes, sensors, and tissue engineering scaffolds.

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Amine-presenting electro-conducting scaffolds

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  1. Amine-presenting electro-conducting scaffolds Jae Young Lee and Christine E. Schmidt

  2. Motivation • Amino-polypyyrole (APPy) [Issue] • Neurons normally adhere poorly to regular PPy films • Conventional adhesion methods involves the external treatment of ‘ECM molecules (laminine, fibronectin, collagen etc’ and ‘ECM fragments (RGD, IKVAV etc)’, or ‘polycationic polymers (Polylysine, PEI, PAA)’. [Beneficial aspects] • APPy has positive charge – possible increase in cell adhesion. • Theoretically permanent display of positive charge on the substrate. • Has potential to be used for interfactial materials between neurons and electrodes (neural probes, sensors, and tissue engineering scaffolds)

  3. Synthesis 1-aminopropyl pyrrole was synthesized from 1-cyanoethyl pyrrole using a reduction reaction with LiAlH4. The reflux reaction was carried out for 20 hours in a round bottom flask in ethyl ether under Ar gas. Purity was confirmed using NMR and TLC (ninhydrin). [1] 1-(2-cyanoethyl)pyrrole [2] 1-(3-aminopropyl)pyrrole

  4. Water Contact Angle • Water contact angle was then measured to see how hydrophibic or hydrophillic the samples were: • Hydrophobic: Poor cell adhesion and destruction of protein activity. • Hydrophillic: charged on sample. Is desired.

  5. Material characterization • Conductivity: 4 pt probe techniques employed • Surface amine: quantified using ninhydrin methods

  6. Cell Adhesion Studies (MTS): Fibroblast

  7. A B C Cell Adhesion Studies: FL images Live staining of fibroblast cultured on the sample: (A) reg. PPy, (B) APPy(50:50), and (C) APPy(100).

  8. Acknowledgement Jae Young Lee Dr. Christine Schmidt Derek Ensign Gregory Abraham

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