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Amine-presenting electro-conducting scaffolds

Amine-presenting electro-conducting scaffolds. Jae Young Lee and Christine E. Schmidt. Motivation. Amino-polypyyrole (APPy) [Issue] Neurons normally adhere poorly to regular PPy films

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Amine-presenting electro-conducting scaffolds

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  1. Amine-presenting electro-conducting scaffolds Jae Young Lee and Christine E. Schmidt

  2. Motivation • Amino-polypyyrole (APPy) [Issue] • Neurons normally adhere poorly to regular PPy films • Conventional adhesion methods involves the external treatment of ‘ECM molecules (laminine, fibronectin, collagen etc’ and ‘ECM fragments (RGD, IKVAV etc)’, or ‘polycationic polymers (Polylysine, PEI, PAA)’. [Beneficial aspects] • APPy has positive charge – possible increase in cell adhesion. • Theoretically permanent display of positive charge on the substrate. • Has potential to be used for interfactial materials between neurons and electrodes (neural probes, sensors, and tissue engineering scaffolds)

  3. Synthesis 1-aminopropyl pyrrole was synthesized from 1-cyanoethyl pyrrole using a reduction reaction with LiAlH4. The reflux reaction was carried out for 20 hours in a round bottom flask in ethyl ether under Ar gas. Purity was confirmed using NMR and TLC (ninhydrin). [1] 1-(2-cyanoethyl)pyrrole [2] 1-(3-aminopropyl)pyrrole

  4. Water Contact Angle • Water contact angle was then measured to see how hydrophibic or hydrophillic the samples were: • Hydrophobic: Poor cell adhesion and destruction of protein activity. • Hydrophillic: charged on sample. Is desired.

  5. Material characterization • Conductivity: 4 pt probe techniques employed • Surface amine: quantified using ninhydrin methods

  6. Cell Adhesion Studies (MTS): Fibroblast

  7. A B C Cell Adhesion Studies: FL images Live staining of fibroblast cultured on the sample: (A) reg. PPy, (B) APPy(50:50), and (C) APPy(100).

  8. Acknowledgement Jae Young Lee Dr. Christine Schmidt Derek Ensign Gregory Abraham

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