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Photofabrication of 3D Protein Hydrogels for Nanobiotechnology Applications

BAYINDIR GROUP JOURNAL CLUB-1. Photofabrication of 3D Protein Hydrogels for Nanobiotechnology Applications. Erol Özgür. MOTIVATIONS. Microfabricated platforms in cellular biology: valuable research tools Microfluidics technology Major problem is biocompatibility

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Photofabrication of 3D Protein Hydrogels for Nanobiotechnology Applications

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  1. BAYINDIR GROUP JOURNAL CLUB-1 Photofabrication of 3D Protein Hydrogels for Nanobiotechnology Applications Erol Özgür

  2. MOTIVATIONS • Microfabricated platforms in cellular biology: valuable research tools • Microfluidics technology • Major problem is biocompatibility • Limited functionality of interfacial materials • No perturbation during experiment process is possible • Aim: Platforms for cellular research that could be modified in situ

  3. Protein Cross-linking for Formation of 3D Hydrogels • BSA (Bovine Serum Albumin) is a soluble protein with 607 residues • BSA(aq) BSA(prec.) • UV light: Inefficient, little control on the shape • Multiphoton excitation: Precisely controlled submicrometer sized shapes • Photosensitizers increase the efficiency of the process • Optical parameters: • Tsunami Ti:Sapphire laser at 730-740 nm, Zeiss Axiovert IM 100X Fluar 1.3 NA objective, with a confocal scanner hν

  4. In Situ Formation of Protein Hydrogel

  5. 3D Protein Hydrogels Created by Photofabrication with Photomasks

  6. Chemical Response of Protein Hydrogels: Na2SO4

  7. Chemical Response of Protein Hydrogels: Na2SO4

  8. Chemical Response of Protein Hydrogels: pH from 7 to 12.2 with NaOH

  9. Microchambers with E. coli Directionality

  10. Microvortex Created by E. coli

  11. Axial Fluid Flow in an Arched Microchannel Produced by Flagellar Motion of E. coli

  12. Orbital Revolution of a PMMA microdisk by E. coli

  13. Different Strategies in Photofabrication With Photomask and Confocal Scanner With Digital Micromirror Device

  14. Fabrication Process with DMD

  15. Some Structures Fabricated with DMD

  16. References Kaehr, B. and J. B. Shear (2008). "Multiphoton fabrication of chemically responsive protein hydrogels for microactuation." PNAS105(26): 8850-8854. Kaehr, B. and J. B. Shear (2009). "High-throughput design of microfluidics based on directed bacterial motility." Lab on a Chip 9(18): 2632-2637. Nielson, R., B. Koehr, et al. (2009). "Microreplication and Design of Biological Architectures Using Dynamic-Mask Multiphoton Lithography." Small 5(1): 120-125.

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