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DNA Barcodes for Assessment of the Biological Integrity of Aquatic Ecosystems

DNA Barcodes for Assessment of the Biological Integrity of Aquatic Ecosystems . Mark Bagley, United States Environmental Protection Agency Charles Spooner, US EPA Ronald Klauda Maryland Dept of Natural Resources David Schindel, Consortium for the Barcode of Life

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DNA Barcodes for Assessment of the Biological Integrity of Aquatic Ecosystems

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  1. DNA Barcodes for Assessment of the Biological Integrity of Aquatic Ecosystems Mark Bagley, United States Environmental Protection Agency Charles Spooner, US EPA Ronald Klauda Maryland Dept of Natural Resources David Schindel, Consortium for the Barcode of Life Lee Weigt, Smithsonian Institution Robert Hanner, University of Guelph

  2. Ecological Integrity Physical Integrity Chemical Integrity Biological Integrity Bioassessment An evaluation of the biological condition of a waterbody using biological surveys and other direct measurements of the resident living organisms

  3. US Wadeable Streams Assessment • National assessment of the condition of wadeable streams • 10 different taxonomic ID laboratories • 749 stream macroinvertebrate samples (sites) • All organisms identified to genus only • 10% random re-identification by independent taxonomist • Data quality objective – 85% repeatability Credible environmental decision-making depends on objectivity and repeatability of taxonomic results

  4. EPA Advanced Monitoring Initiative Project Goals • Develop a DNA barcode library for important aquatic indicator species (EPT) • Ephemeroptera (Mayflies) • Plecoptera (Stoneflies) • Trichoptera (Caddisflies) • Compare DNA barcodes to traditional bioassessments for EPT taxa • Cost, Speed, Objectivity, Accuracy, Precision • How important is increased taxonomic precision? • Determine how to efficiently incorporate DNA barcodes into a state bioassessment program

  5. Repeatability and barcode development Reference Barcode Database Maryland DNR Guelph (taxonomic agreement) Smithsonian (Adult Voucher specimens) EPA Lab EPA (disagreement or MOTU) Taxon Experts Morphology DNA Repeatability Accuracy Precision Cost Repeatability Precision Cost Species Description

  6. Tech Transfer is a Major Project Goal • End users are participants in the project • Maryland DNR, EPA-Water • Tech transfer documents, hands-on workshops, and protocols are key products • Chose influential end-users that will “convert” others

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