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RF-C is a five-subunit complex All subunits are related in sequence and have ATP binding motifs

RF-C is a five-subunit complex All subunits are related in sequence and have ATP binding motifs ATP hydrolysis by RF-C is associated with the loading of PCNA RF-C is the functional homolog of the clamp-loader g complex. Polymerase switching occurs even on lagging strands;

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RF-C is a five-subunit complex All subunits are related in sequence and have ATP binding motifs

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  1. 免疫学信息网http://www.immuneweb.com

  2. 免疫学信息网http://www.immuneweb.com

  3. 免疫学信息网http://www.immuneweb.com

  4. 免疫学信息网http://www.immuneweb.com

  5. RF-C is a five-subunit complex All subunits are related in sequence and have ATP binding motifs ATP hydrolysis by RF-C is associated with the loading of PCNA RF-C is the functional homolog of the clamp-loader g complex 免疫学信息网http://www.immuneweb.com

  6. 免疫学信息网http://www.immuneweb.com

  7. Polymerase switching occurs even on lagging strands; pol d does most of DNA synthesis 免疫学信息网http://www.immuneweb.com

  8. 免疫学信息网http://www.immuneweb.com

  9. How do you think the PCNA is removed after ligation? 免疫学信息网http://www.immuneweb.com

  10. 免疫学信息网http://www.immuneweb.com

  11. PCNA interacts with RF-C, pol d, Fen1, DNA ligase, CAF1 and MCMT Several of these have a common motif used in the interaction: Q-X-X-L/I/M-X-X-F-F/Y p21/CIP1/WAF1, a protein induced by the tumor suppressor p53 uses the same motif to interact with PCNA What effect is p21 expected to have on DNA replication/repair? 免疫学信息网http://www.immuneweb.com

  12. FEN1 interacts with PCNA 免疫学信息网http://www.immuneweb.com

  13. DNA Helicase * Helicase binds ss DNA * Hydrolyzes ATP to move along ssDNA and peel of substrate DNA * Can move 5‘ to 3’ or 3‘to 5’ or in both directions on ssDNA and is classified as such * Dimers or hexamers 免疫学信息网http://www.immuneweb.com

  14. Helicases are ATP driven molecular motors Conserved helicase motifs: Includes the following for ATP binding and hydrolysis I = Walker A motif GxGxGKT II = Walker B motif FFFDEad 免疫学信息网http://www.immuneweb.com

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