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?????S??????s??p?????ta? ??a: t?? ?p?st????? ?a? sta?e??p???s? ?a???, ?a??????, ??p?es??, t?? ?p?st????? ????? ? ?????s??, t?? pe?????s? t?? ????t???t?ta? (a????t?p???s?), t?? pe?????s? ??d?at??, t? e??s? t?? p????.. ??????????? ?????S??????s??p?????ta? ??a ste???s? ep??e?t??, ??e
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1. ??T???: ??O??S ???T???S ?????S??? ??? ???T????? ?. ?IEKKAS
2. ?????S???
???s??p?????ta? ??a:
t?? ?p?st????? ?a? sta?e??p???s? ?a???, ?a??????, ??p?es??,
t?? ?p?st????? ????? ? ?????s??,
t?? pe?????s? t?? ????t???t?ta? (a????t?p???s?),
t?? pe?????s? ??d?at??,
t? e??s? t?? p????.
3. ??????????? ?????S???
???s??p?????ta? ??a ste???s? ep??e?t??, ??e??? a????a??a?, pe?????s? ????t???t?ta?, ?p?st????? d?ast?e?t??.
??p??:
- d??t??t??: a?a?? p???? (ae??s?? p?????), a?at??????? ??a t?? ?s??s? p?es?? ? t?? ?p?st????? a????se??,
- ep?des?? e e????? p??sa???? st? s??a t?? ?????: ste???s?, ?p?st?????,
- ep?des?? ap? e?ast??? ?????: ?p?st????? se a????se?? (ep????at?de?, pe??sf???de?).
4. ??????S ?F????G?S ??????????? ?????S??? (1)
Sta?e?te p??st? ap? t? t?a?a.
??ate?ste t? ???? st? ??s? p?? ?a pa?ae??e? et? t?? ep?des?.
??ate?ste t?? ep?des? ?ts? ?ste ? ep?f??e?a p?? ?a a???p? st? t?a?a ?a ?? ??e? ???e? se epaf? e t? ???? sa? (a?t? p?? t?? ??at?e?).
? ep?des? ?e???? t?p??et??ta? t?? ???? t?? ep?des?? st? t?a?a.
5. ??????S ?F????G?S ??????????? ?????S??? (2)
G????ta? d?? a?????? pe??st??f?? ??a ?a ste?e??e? ? ep?des?? ?a? st? s????e?a t????eta? spe???e?d??, ap? t?? es?te???? p??? t?? e??te???? p?e???.
?et? t?? t?p???t?s?, ???e 10 ?ept?, e????eta? ? ?????f???a t?? a?at??, ?d?a?te?a a? ???s??p?????ta? ep?des?? p?? p??sa?????ta? st? s??a t?? ????? (p?????s? ??d?at??).
6. ???O???? ?????F???? ??????S
?? d??a t?? ????? ???eta? ???? ?a? ?????te?? ap? t? ?p????p? d??a.
? as?e??? d?aa?t??eta? ??a ??d?asa ?a? ad??a?a ????s??.
???????O??S?
?et????te t?? ep?des? ???? ?a epa????e? t? ???a ?a? ? ?e????as?a t?? d??at??.
????ete e t? ???? st?? p????at??? pe????? ???? ?a asp??se?. ?ta? t? af?sete, t? ???a p??pe? ?a epa????e? a?s??. ?a?at????te t?? ep?des?.
7. ??G?T? ??????????? ?????S???
? ep????? e?a?t?ta? ap? t? ???? st? ?p??? ?a ???s??p????e? ?a? p??pe? ?a e??a? p??? p?at?te??? ap? t? t?a?a:
2,5 cm ??a t? d??t???,
5 cm ??a t?? ???a ?e??a,
7,5-10 cm ??a t? ?a????a,
10-15 cm ??a t? p?d?
8. ?????? ?????S?S ??????????? ?????S???
9. ?????S? ?G?O?? G?????S
??? ????? ?????? ??????
- Sta?e??p???s? ep??e?t?? ? ?p?st????? t?a??t?? a?a??? ????? (d?ast??ata) ? ?ata??t??.
- ?pa?te?ta? fa?d?? ep?des?: ? t?p???t?s? t?? ep?d?s?? ?a e?te??eta? p??a ap? t?? ?????s?.
- S??p??: ? ?s??s? epa????? p?es?? st?? t?a?at?s??? pe?????.
10. S?????? se ?at??ata ?a??????? ?a? ?e???da?.
???d???? t??s?? a??e???-?e????: apa?a?t?t?? ? ??e???? t?? ?????f???a?.
14. ???esa sta d?? p?d?a t?p??ete?ta? apa?a?t?ta ?a pets?ta d?p????? se ?????d???? s??a (p????? a????t?p???s?).
? ??p?? ?at? p??t??s? t?p??ete?ta? st? e?d??es? ?e?? t?? p?d???.
??a??a?t???, efa???? ?f?sat?? p???? t?? p????? (sa? a???a).
15. S????? st??? ???????????.
??s?? p?s???t?? ????? e t? ????? (a????t?p???s?).
??? st??f? ???at??-p?d???: e???????s? p?d??? e ???? ?at? t?? ep???? ????a.
?p?des? st? ???? ????, ????t??, ast?a?????.
16. ??s?? p?s???t?? ????? e t? ????? (a????t?p???s?).
????????s? p?d??? e ???? ?at? t?? ep???? ????a.
?p?des? st? ???? ????t??, ast?a?????.
17. ???GO????? ?????S???
?p?????? ?t???? se ap?ste?????e? s?s?e?as?e? ? ?atas?e?????ta? d?p?????ta? ? ????ta? ??a tet?????? a??e?t??? ?fasa d?a????a.
???s??p?????ta? ??a ste???s? ep??e?t?? ? ??????a, ?? a?a?t??e? ? p?ate?? ep?des??, ??a t?? a????t?p???s? e??? ?a? t?? ?p?st????? d?ast?e?t??.
18. F????S ??? S????S ???GO????S ?????S??S
19. S???????????
20. ?????S? ????? ?????? - ??????
21. ?????S? ????O??? ??F???S
22. ?????S? ??????S
23. ?????S? O???
24. ?????S? TO???? - ????S
25. ?????S? G?????? - ?S????
26. ????????S ?????S???
???s??p?????ta?:
??a ?a ?p?st??????? t?a?at?s??? ?a????a ? ?a?p?,
??a ?a e??s??? t? ???? t?? ?a????a ap? e?a??????? ??,
??a ?a a????t?p???s??? t? ?a????a ep? ????s?? t?? d??????s?? t?? ???,
se t?a?ata st? ????, ??a t?? ??e??? t?? a????a??a? ?a? t?? pe?????s? t?? ??d?at??.
? as?e??? p??pe? ?a e??a? se ?a??st? ??s? ?a? ?a ?p?st????e?, a? p??e?, t?? t?a?at?s??? ?a????a.
27. ?F????G? ???????? ?????S??? (1)
28. ?F????G? ???????? ?????S??? (2)
29. ????S?????? ????????S ?????S???
30. S??????S??S ?????????????S ?????S???
31. ????T???S? ?????????????S ?????S??? S? ??????? ????G??
32. ????S???? ????S?????? ???????? ??????
33. ???OS??S S?????????S S????S
34. ?F????G? G????O? ?????S?O?
?fa???eta? se e?d??es? st??a ?a??? (p??stas?a d??at??),
? ????ta???a t?p??ete?ta? ?p? t?s?, ?p???e????ta? t??? ?st??? se e?af?? ?p?????s?,
S?pe???a????ta? ?? e?at????e? t?? ?at??at?? a????se?? (e?t?? ?e???da?-?????), ?? ?p??e? a????t?p?????ta? se ?e?t??????? ??s?,
?a ?e??a p??state???ta? e p??s?et? st??a ?a???,
?fa??es?: ap?f??? t?a?at?s?? t?? d??at??. ?? ????p????? (e?te?e? pa???? ????s?) de? p??pe? ?a s??eta? ?at? ???? t?? ????? (pa???s?a ?a???).
35. ?????????T?S? ?ST??O? ?? G????? ?????S??
??????p? ??s? ??t? ????? ? a???t?s? ??? ????? ap? t?? a????a (?p?????s? f?e???? epa?af????),
??e?e???s? ?a? a?t?et?p?s? p????,
??e???? ?????f???a? t?? ?????: ? ?????s? ????? ?p?d????e? f?e??? ????a, e?? ta ?e??? ?a? ????? ???a a?t???a?? ???, a?s??t???t?ta d??at??,
???t??a: e??tas? ??a ??d?a, e???????s? t?? as?e?? ?a e?te?e? ????se?? (f?e??? ep?st??f?), ??e???? pa??t???? ????s?? (p????: ?s?a??a t?? ?apt???? ???),
??e???? e?e???se?? d??at?? (p?es? st?? es?te???? ep?f??e?a t?? ?????): t?p???? p????, d?s?s?a, e????, p??et??,
???a?? ? afa??es? ?????.
36. ???T?????
???s??p?????ta? ??a:
t?? ??e??? t?? a????a??a? (a??stas?),
t? e??s? t?? ???d???? ep?????s?? t?? p?????,
t?? p??a???? t?? ep????s?? (p??stas?a ?e?s??at???e??? ep?????a??? ?st??),
t?? e?asf???s? ? t?? ap??????s? (ap????f?s?) t?? ???as?a?,
t? e??s? t?? p????.
37. ??????S ?F????G?S ???T????O? (1)
???s??p???ste ap?ste?????a ???t?a ??a t?? p?????? et?d?s?? ?????e??.
?a?a??ste p??se?t??? t? t?a?a: ap?a????ete ???a s?ata ? ?e????? ?st???.
??p??et?ste a?t???t??? a???f? st?? es?te???? ep?f??e?a t?? ep???at??.
?? ep??ea p??pe? ?a epe?te??eta? p??a ap? ta ???a t?? p????? (p????? ??????) ?a? ?a ?? ??e? ???e? se epaf? e ???e? pe??????.
?? d?ap?t?ste? ap? a?a, t?p??ete?ste de?te?? ep??ea p??? st? p??t?.
38. ??????S ?F????G?S ???T????O? (2)
??? a????ete e ta ????a t?? p???? ? t?? es?te???? ep?f??e?a t?? ep???at??, ap?f??ete ?a ??ete, ?a fta???ste?te ? ?a ???sete p??? ap? t?? p????.
Ste?e?ste e t?? efa???? ep?d?s?? ? ad??????? p?ast???? a? ? pe????? a?a??eta? ?a e?te?e? se ?e??.
?a ????? p?? ???s??p??????a? t?p??et???ta? se p?ast??? sa????a p?? ?atast??feta? se ???a??.
??t?ste t?? as?e?? ??a pa???s?a p????, sf???at??, a? a?s???eta? t?? pe????? ?a? a? p??e? ?a ????se? t? ????.
39. ??????S ????G?S ???T????O? (1)
?????ste t? d?ad??as?a st?? as?e??.
S???e?t??ste ta ?????. ? a??a?? de? apa?te? s?????? d?ad??as?e? ap?ste???s??.
?f?? f???sete ap?ste?????a ???t?a, afa???ste t? ep??ea. ?? ??e? ?????se?, ? ????s? f?s????????? ???? t? ???? ?a ?a?a??se?.
???????e?ste t?? p???? ??a s?e?a ??????? (e????, d?s?s?a) ?a??? ?a? ??a t?? p???d? t?? ep????s??. ??ete ?p??? t? d?sf???a t?? as?e?? ?at? t? d?ad??as?a.
40. ??????S ????G?S ???T????O? (2)
?a?a??ste t? t?a?a e f?s???????? ???.
?????te ap?ste?????a ???t?a.
??p??et?ste t? ??? ep??ea ?a? ste?e?ste t? ?at?????a.
?a ????? p?? ???s??p??????a? t?p??et???ta? se p?ast??? sa????a p?? ?atast??feta? se ???a??.
?ata????te ?at?????a t? d?ad??as?a ?a? p??a??? pa?at???se??.
41. ????? ???T????O? (1)
G??e?: ap?ste?????e? ?fas?t??e? d??? ap????f?t???? ?a??? ? s???et???? ??????, ???s??p?????ta? ?d?a?te?a st? ?e????????? (a??? t?a?ata).
??t??????ta: ???a ? ??tta???? p??? se a?t??????t? ?????, ???s?a ??a ????? p????? (e?d??? ??a a????e?, ft???e?, d??t??a).
?d???????e?d?, ?d?????d?: a?t??????ta, ??a e??ss??a e??a?ata, ???? ?ata???se?? ?a? t?a?at???? ?a??se??, s??at????? gel ?a??? ap????f??? ???? ap? t? t?a?a.
Hydrogels: ?????? ??a ???s??a t?a?ata ? e ?e????? ?st???, ??? se t?a?ata e pe?????s??? ?????s? ????? (af?d?t?s?).
42. ????? ???T????O? (2)
?d??p???e??: d?a??t??? p???ap?? st??ata, ap????f??? ???? ?a? d?ast?????ta? ?ste ?a ?a?????? t? t?a?a.
?pe?ap????f?t???: ?????? ????t???t?ta?, ta?e?a ap????f?s?.
???????e?????: d?a??t??? ??????, a????t??? ?a????? p?? ap????f??? ????.
???s???et???, ????a?????: ?atas?e?????ta? ap? ??????? ?st??? ?a? p???e??. ???s??p?????ta? se e??a?ata ?a? d?s???a st?? ep????s? t?a?ata a?t?st???a.
43. ????? ???T????O? (3)
??afa?e?? e???e?: de? ??????? se ????? ep?f??e?e? ?a? ap?t??p??? t?? e?s?d? a?t????? ?a? ????? st? t?a?a (ep? ?????? ???d???? ???????).
?e pa?????te? a??pt????: ep?d???? se s???e?????a ??tta?a ep?ta?????ta? t?? ep????s? (d?a?t??? p?d?).
?p??at?stata d??at??: p???????ta? ap? a????p??a ??tta?a p?? ?a???e?????ta? ?a? d?ast?????ta? in vitro (?????? ??a f?e??? ???? p?d???).
44. ??????????? ???T???
45. G???
46. HYDROGEL
47. ????????S??S
?a ep???ata p??pe? ?a ???s??p?????ta? e p??s??? st?? pe??pt?s? pa???s?a? Gram (-) a?t????? (af?????? se pe???????ta ?????? ???as?a?) ?a? se as?e?e?? e a??s??atast???. S?a?t??? ? s???? a??a?? t?? ep??e?t??.
Hydrogel: e?????? t?? a??pt??? Gram (-) a?t????? st?? pe??pt?s? p?? a?t? e?s?????? st? t?a?a.
Hydrogel ?a? ?d???????e?d?: ?da???? ??a a??? t?a?ata: ap????f??? ta ???? p?? e???????ta?, e?asfa?????? ???as?a apa?a?t?t? ??a t?? ep????s?, p??sf????? ??es? st?? as?e??.
???????e?????: ?da???? ??a t?a?ata p??s?p??: e?t????? a?s??t??? ap?t??esa (pe?????s?? ????d??? ?st??).
48. ??????S ?F????G?S ???T????O? S? ?G????????S
??a?at?p??e?ta? et? t? ??????s? a?a???s?a?.
F???ste ep?p???? ????a a????? ?a? ?e????????? ?s?a.
? s?et??? t?? as?e?? st? d?ad??as?a t?? pa???e? ??p??? a?? e?????? st?? p???.
???a?? ?a ??e?ast??? ?a??d?a ?a? ?a?de? ??a t?? afa??es? t?? ep??e?t?? ?a? t?? ?e???t???? es???a?.
???????e?ste t?? e??a?at???? pe??????, ?a?a??ste ?at?????a ?a? t?p??et?ste t?? apa?a?t?te? a???f??.
?? ???a?a ?a??pteta? s?????? e p???ap?? st??ata ep??e?t??.
? t?p???t?s? t?? ep???at?? p??pe? ?a ep?t??pe? t?? ????s? t?? ????? (e?t?? a? a?t? a?te?de????ta?).
