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1. 4. Materials & Methods. Korean domestic pig (♂). Korean domestic pig ( ♀ ). Large White(♂). Large White ( ♀ ). 3. 2. F1( ♀ )-KY. F1( ♀ )-YK. F1 (♂)-YK. F1 (♂)-KY. Identification and Characterization of PERV Elements in Different Pig Genome

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  1. 1 4 Materials & Methods Korean domestic pig (♂) Korean domestic pig (♀) Large White(♂) Large White (♀) 3 2 F1(♀)-KY F1(♀)-YK F1 (♂)-YK F1 (♂)-KY Identification and Characterization of PERV Elements in Different Pig Genome HUH Jae-Won1, KIM Dae-Soo2, CHO Byung-Wook3 and KIM Heui-Soo1,2* 1Division of Biological Sciences, College of Natural Sciences, Pusan National University.   2PBBRC, Interdisciplinary Research Program of Bioinformatics, College of Natural Sciences, Pusan National University.   3Department of Animal Science, College of Life Sciences, Pusan National University. http:// www.primate.or.kr Abstract Results & Discussion PERV LTR structure • Porcine endogenous retrovirus (PERV) released from pig cells is a main risk factor associated with xenotransplantation from pig to human. Overcoming the main obstacle for xenotransplantation, the character of PERV element must be fully understood. From now, PERV have been identified to have three classes : PERV-A, PERV-B, PERV-C. PERV-A and PERV-B have been reported to have a infection ability for specific human cell line. However, PERV-C could not replicate their genome in human cell line. Using bioinformatic tools, several PERV long terminal repeat (LTR) elements were identified and analyzed from Genbank database sequences.Various subtype of PERV LTR elements were analyzed by the bioinformatic tools. Some of PERV LTR elements show a highly conserved sequences and insertional polymorphism. We cloned different subtype of LTR elements and analyzed the expression pattern from Yorkshire, domestic pig, and domestic wild pig.…………………………………….……………………… Expressed PERV LTR structure Introduction Methylation analysis of PERV LTR elements PERV-A7 PERV-A5 PERV-X9 PERV-A8 Promoter and phylogenetic analysis of PERV LTR elements • Materials • Yorkshire • Hybrid pig (YorkshireⅹKorean domestic pig) • Sus scrofa coreanus • Methods • Genbank database mining • 2. Genomic PCR and RT-PCR analysis • 3. Gene cloning and sequencing • 4. Methylation analysis • 5. Promoter analysis References • Le Tissier P, Stoye JP, Takeuchi Y, Patience C, Weiss RA (1997) Two sets of human-tropic pig retrovirus. Nature 389: 681-682. • Patience C, Takeuchi Y, Weiss RA (1997) Infection of human cells by an endogenous retrovirus of pigs. Nat Med 3: 276-282. • Scheef G, Fischer N, Krach U, Tonjes RR (2001) The number of a U3 repeat box acting as an enhancer in long terminal repeats of polytropic replication-competent porcine endogenous retroviruses dynamically fluctuates during serial virus passages in human cells. J Virol 75: 6933-6940 Sus scrofa coreanus GENOME INFORMATION LABORATORY

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