AFB Microscopy

Oct 07, 2012

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----- smear contains no AFB in 100 fields.. NEG. How will you grade a smear with 9 AFB in 100 fields ?. . positive(Actual) 09 AFB. ------ Smear with more than 10 AFB per field.. 3 . A smear with 1 to 9 bacilli in majority of the fields will be graded as:. 2 . Smear grade: 3 . ( >10 AFB/f

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AFB Microscopy

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External Quality Assessment EQA for AFB Microscopy

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The following points are true regarding AFB smear microscopy

False positive AFB smear results are usually due to: . Usually poor quality microscopePoor stain quality (basic fuchsin-filtration)Technician does not recognize morphology of AFBAll of the aboveNone of the above. The sensitivity of AFB smear microscopy depends on:. Quality and number of sputum s

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Microscopy

Microscopy. An important means of studying MICROANATOMY . Overview of discovery. Abbe & Zeiss. Campani. George Bass. Chester Hall. Pasteur. Amici. Dollond. Lister. Geroge Adams. From the Bioscope Initiative with permission. Dissecting Microscope.

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Microscopy

Microscopy. MICROSCOPE 18th Century. A compound microscope used in 18th-century France. First developed in the late 16th century, compound microscopes use two or more sets of lenses to achieve high magnification. TYPES OF MICROSCOPE. I. OPTICAL MICROSCOPES. Hooke’s Microscope.

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Module 10: Quality Assurance of AFB Microscopy

2. Learning Objectives. At the end of this module, you will be able toDescribe the elements of Quality AssuranceExplain why internal Quality Control is important to assessing laboratory performanceDescribe the three components of External Quality AssessmentPrepare for a supervisory visitDescrib

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AFB-MICROSCOPY FOR TB DIAGNOSIS

AFB-MICROSCOPY: SPECIFICITY. Over 99% for AFB. SPECIFICITY OF AFB SMEARS FOR TB . REFERENCE / COUNTRY HIV AFB PATHOGENS, NOT TBLong, Haiti, 1991 26%4/199 patients(2/2 HIV /-)Githui, Kenya, 199233%0/320 patientsWarndorff, Malawi, 1997 >50%?2/904 patients(1/1 HIV /-)Van Deun, Bangladesh, 2001 <1%3/1065 patients.

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LUKE AFB AIRSPACE

LUKE AFB AIRSPACE. SUNNY MOA & ATCAA ACTIVE SUNRISE - 2300(L) MON-FRI 12000’ MSL - FL290. GREEN = MILITARY OPERATIONS AREA RED = RESTRICTED AIRSPACE MAGENTA = ALERT AREA BLUE = MILITARY TRAINING ROUTES. ALERT AREA A-231 ACTIVE SUNRISE - 2300(L) MON-FRI 500’ AGL - 6500’ MSL

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Microscopy

Microscopy. How to Properly Prepare and Use a Microscope. Parts of the Microscope. Eyepiece (ocular) Body tube/Eyepiece tube Revolving nosepiece Objectives (lens) Stage Clips Newer microscopes have mechanical knobs that move the slide Diaphragm Base Light Source Fine Adjust/Focus

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Microscopy

Microscopy. Introduction of Microscopes. Microscopic – invisible to naked eye 1595 Magnified images approximately 3x – 10x. Compound Microscopes. Uses natural or artificial light to see objects. Parts of a Compound Microscope. Ocular lens Objective lens Low, Medium, and High power Body

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Microscopy

Microscopy. Micro = ‘very small’ Scope = ‘to see’ Early biologists could only examine what they could see with the naked eye. Middle ages : Used a single lens magnifying glass (10-30X power) 1590: Two Dutch eyeglass maker – Hans and Zacharias Janssen Made the first compound microscope

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Module AFB MICROSCOPY

Module AFB MICROSCOPY. Content Overview. Collection of sputum specimens Quality of sputum specimens Processing of sputum specimens Registration of sputum specimens Sputum smearing and staining Smear examination Recording and reporting. Collection of Sputum Specimens.

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MICROSCOPY

MICROSCOPY. What do microscopes do?. An optical instrument that uses a lens or a combination of lenses to produce magnified images of small objects, especially of objects too small to be seen by the unaided eye. http://www.answers.com/microscope micro scope.

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MICROSCOPY

MICROSCOPY. "micro" (small) "scopeo" (to watch). THE RELATIVE SIZES OF MOLECULES, CELLS AND ORGANISMS. THE RELATIVE SIZES OF MOLECULES, CELLS AND ORGANISMS. MICROSCOPY. MICROSCOPY. 1590. 200 9. THE LIGHT.

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Microscopy

Microscopy. UNITS OF MEASUREMENT. 1mm = 1000µm 1µm = 10 -3 mm (convert mm to µm by multiplying by 1000 = 3 zeros) Bacteria are about 1µm or smaller 1nm = 10 -6 mm (convert nm to mm by dividing by 1000000 = six zeros) Viruses are about 1nm 1000nm = 1µm

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Microscopy

Microscopy. What is a microscope?. A scientific tool that helps scientists see things that cannot normally be seen with the “naked” eye. History. Hans and Zacharias Janssens-1590s, Dutch Eyeglass Makers, Inventors Early microscope was composed of a tube with lenses at both ends

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Microscopy

Lab Exercise # 1. Zoo- 145. Microscopy. OBJECTIVE: STRUCTURE, FUNCTION AND OPERATION OF LIGHT MICROSCOPE. التركيب والوظيفة وطريقة التشغيل. Lab Exercise # 1. Zoo- 145. INTRODUCTION.

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Microscopy

Microscopy. Looking at the small stuff. Microscopes. “micro” means : Small “scope” means: Watch/ look It was actually developed first as entertainment for the rich. Light Microscope. Focuses visible light rays Can be used on live or dead specimens

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Microscopy

Microscopy. Microscopes. There are different kinds of microscopes: the light microscope the electron microscope. Resolution and Magnification. The amount of detail in an object that can be seen is called “resolution”. Examine the four pictures of the fly’s head.

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MICROSCOPY

MICROSCOPY. Tutorial 1. Types of Microscopy. Light Fluorescence Confocal Electron Transmission Electron Microscopy (TEM) Scanning Electron Microscopy (SEM). LIGHT MICROSCOPY. Advantages Live cells can be viewed, color can be seen Disadvantages Limit of resolution is 0.2 microns

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Microscopy

Microscopy. Light and Electron Microscopy. The First Light Microscopes. Around 1590 Zaccharias and Hans Janssen experimented with lenses in a tube, leading to the forerunner of the microscope and the telescope

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MICROSCOPY

MICROSCOPY. Microscopy is the technical field of using microscopes to view samples or objects. There are three well-known branches of microscopy, optical, electron and scanning probe microscopy.

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