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Immunology (elective) MLIM-101

Immunology (elective) MLIM-101. Prepared by: Dr. Mohamed S. Abdel- Latif. Immunoglobulin: Isotypes , Allotypes and Idiotypes. Learning Outcome:. At this time you should know the following: 1. To explain the structural basis for immunoglobulin isotypes , allotypes and idiotypes .

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Immunology (elective) MLIM-101

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  1. Immunology (elective)MLIM-101 Prepared by: Dr. Mohamed S. Abdel-Latif

  2. Immunoglobulin:Isotypes, Allotypes and Idiotypes

  3. Learning Outcome: At this time you should know the following: 1. To explain the structural basis for immunoglobulin isotypes, allotypes and idiotypes. 2. To describe some of the uses of isotypes, allotypes and idiotypes.

  4. Immunoglobulin Isotypes • Definition - Antigenic determinants that characterize the classes and subclasses of heavy chains and types and subtypes of light chains

  5. Immunoglobulin Isotypes Human IgM Reacts with IgM, IgG, IgA, IgD, IgE ?? antiserum React with IgG, IgA, IgD and IgE ?? Absorbed antiserum Reacts only with IgM Recognizes determinants specific for  heavy chains (isotypes) ??

  6. IgG1 (kappa) IgM (kappa) Immunoglobulin Isotypes • Definition • Occurrence • Importance • Ig levels • B cell tumors • Immunodeficiencies • Location

  7. IgG1 (kappa) Person 1 IgG1 (kappa) Person 2 Immunoglobulin Allotypes • Definition - Antigenic determinants specified • by allelic forms of the Ig genes • Source of anti-allotypic Abs • Location • Occurrence

  8. Human Immunoglobulin Allotypes • Nomenclature • G1m(3) • Km(1)

  9. Human Immunoglobulin Allotypes • Genetics (alleles) • Co-dominant • autosomal genes Km(1)/Km(3) X Km(1)/Km(1) Km(1)/Km(1) and Km(1)/Km(3) • Allelic exclusion

  10. Human Immunoglobulin Allotypes • Genetics (pseudoalleles) • Co-dominant G1m(17) G1m(1) 214 355-358

  11. Immunoglobulin Allotypes • Definition • Location • Occurrence • Importance • Monitoring bone marrow grafts • Forensic medicine • Paternity testing

  12. Immunoglobulin Idiotypes • Definition - Unique antigenic determinants present on individual antibody molecules or on molecules of identical specificity

  13. Immunoglobulin Idiotypes Strain A Mouse Anti-DNP Ab DNP-BSA Ab against combining site of anti-DNP Ab Strain A Mouse Purified anti-DNP Ab ?? Antigenic determinants created by the HVR = Idiotypes

  14. Immunoglobulin Idiotypes • Definition • Location IgG1 (kappa) Person 1 anti-A IgG1 (kappa) Person 1 anti-B

  15. Immunoglobulin Idiotypes • Definition • Location • Importance • V region marker • Regulation of immune responses

  16. Regulation by Id/anti-Id + + Ag Id anti-Id anti-anti-Id - -

  17. Immunoglobulin Idiotypes • Definition • Location • Importance • V region marker • Regulation of immune responses • Vaccines

  18. + + Ag Id anti-Id Ag Id anti-Id - Anti-Idiotype Vaccines

  19. Immunoglobulin Idiotypes • Definition • Location • Importance • V region marker • Regulation of immune responses • Vaccines • Treatment of B cell tumors (T cell)

  20. Assignment: As a part of the semester activity, group of students are selected every week to prepare a short seminar about his/her point of interest in one of the lecture topics. That to be discussed and evaluated during the next lecture.

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