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Mathematical Model of the Saccharomyces cerevisiae cell cycle:

Mathematical Model of the Saccharomyces cerevisiae cell cycle:. Aaron McCalister Graduate Mentor- William Ainsworth Department of Chemical Engineering Louisiana State University Baton Rouge, LA. Mentorship. Mentor – Dr. Benton Ph.D., University of Wisconsin-Madison, 2007

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Mathematical Model of the Saccharomyces cerevisiae cell cycle:

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  1. Mathematical Model of the Saccharomyces cerevisiae cell cycle: Aaron McCalister Graduate Mentor- William Ainsworth Department of Chemical Engineering Louisiana State University Baton Rouge, LA

  2. Mentorship • Mentor – • Dr. Benton Ph.D., University • of Wisconsin-Madison, 2007 • Graduate student- • William Ainsworth • B.S in Biochemistry, Louisiana State University, 2008

  3. Important Literature F. Sherman, Getting started with yeast, Methods Enzymol. 350, 3-41 (2002). Bracht, Arndt et. al Yeast functional analysis reports, Additional modules for versatile and economical PCR-based Gene Deletion and modification in SaccharomycescerevisiaeYeast .14, 953-961 (1998)

  4. Yeast Cells • Eukaryotic • Gene Sequences are well known • They are inexpensive to maintain • They reproduce at a quick rate • Cell response to DNA damage or other diseases is similar to humans.

  5. Project Goals • To understand the yeast cell cycle by finding the amount of regulatory proteins that are present in different parts of the cell cycle • Develop a model to predict regulation of the Eukaryotic Cell cycle • Once understood, manipulate the cell environment to create a cancerous state. • Then determine ways to treat the cancerous cell.

  6. Polymerase Chain Reaction • (PCR) is a technique in molecular biology used to amplify a single or few copies of a piece of DNA.

  7. Polymerase Chain Reaction is comprised of 3 steps. Denaturation is the first regular cycling event consisting of heating the reaction. The Annealing stage lowers the temperature. During Elongation the DNA polymerase synthesizes a new DNA strand complementary to the DNA template strand.

  8. Gel Electrophoresis Is used for the separation of DNA,RNA, or protein molecules using an electric field applied to a gel matrix.

  9. Agarose Gel As a gel, an agarose medium is porous and therefore can be used to measure microorganism motility and mobility. Factors such as Conformations of a DNA plasmid, and length of the DNA molecule can determine how fast and how far a band moves. Ethidium Bromide is a common dye. It fluoresces under UV light when intercalated into DNA.

  10. Objectives for Summer • Proper laboratory techniques and Protocols • Literature review • Instrumentation familiarization • Yeast Cell Cycle

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