Chapter 10 Recombination / 重组

1. Chapter 10 Recombination / 重组 10.1 同源重组 10.1.1 交换机理 10.1.2 双链断裂 修复机理 10.1.3 RecBCD途径 10.1.4 基因转换 10.2 非同源重组 10.2.1 转座子 10.2.2 反转录转座子 10.2.3 λ噬菌体的整合 10.1 Homologous Recombination 10.1.1 Mechanism for Crossing-Over 10.1.2 Mechanism for Double- Stranded Break Repair 10.1.3 The RecBCD pathway 10.1.4 Gene Conversion 10.2 Non-homologous Recombination 10.2.1 Transposons 10.2.2 Retrotransposons 10.2.3 Bacteriophage λ integration

2. 10.1 Homologous Recombination 同源重组 Diploid (2n) 2 copies of each chromosome

3. Homologous Recombination

4. If these chromosomes were left unchanged … Identical Child Grand- child

5. Crossing-over in meiosis Different Child Grand- child

6. Homologous Recombination in DNA repair Homologous chromosomes

7. 10.1.1 Mechanism for Crossing-Over / 交换机理

8. Mechanism of recombination in yeast (1/2)

9. Mechanism of recombination in yeast (2/2) Holliday junctions

10. Branch migration of Holliday junctionsHolliday交叉的分支迁移

11. Resolution of the Holliday junctionHolliday交叉的拆分 Resolution of the Holliday junctionto create a crossover产生交换的Holliday交叉拆分 Resolution of Holliday junctionsfor non-crossover不产生交换的Holliday交叉拆分

12. Resolution of the Holliday junctionto create a crossover产生交换的Holliday交叉拆分

13. 10.1.2 Mechanism for Double-Stranded Break Repair / 双链断裂修复机理

14. Resolution of Holliday junctionsfor non-crossover不产生交换的Holliday交叉拆分

15. Brca1/Brca2 deletion and breast cancer Brca1/Brca2缺失与乳腺癌 www.harthosp.org/cancer/breast.htm

16. The roles of BRCA1 and BRCA2 in DNA repair BRCA1和BRCA2在DNA修复中的作用 www-ermm.cbcu.cam.ac.uk/01003118h.htm

17. Now showingMeselson-Radding Heteroduplex Model File: biophoto7 \ Introduction to Genetic Analysis \ Meselson-Radding heteroduplex Model

18. 10.1.3 The RecBCD Pathway (1/3)

19. The RecBCD Pathway (2/3)

20. The RecBCD Pathway (3/3)

21. 10.1.4 Gene Conversion / 基因转换 Homologous, Not identical. G C A T 3’ 5’ 3’ 5’ G T A T 3’ 5’ 3’ 5’

22. Gene Conversion / 基因转换

23. Gene Conversion / 基因转换

24. Neurospora crassa 粗糙脉孢菌 + + 4 : 4 + + + + + + + + m m m m m m m m m Meiosis I 减数分裂I Meiosis II 减数分裂II m Mitosis 有丝分裂

25. Neurospora crassa 粗糙脉孢菌 4 : 4 6 : 2 5 : 3 www.neurosporagenome.org/ www.nigms.nih.gov/Initiatives/Models/ www.neurosporagenome.org/

26. Now showingGene Conversion File: biophoto7  Introduction to Genetic Analysis  Genetic Consequences of Meselson-Radding Model

27. 10.2 Non-homologous Recombination非同源重组 10.2.1 Transposons 转座子 10.2.2 Retrotransposons 反转录转座子 10.2.3 Bacteriophage λ integration λ噬菌体的整合

28. 10.2.1 Transposons / 转座子

29. Transposition / 转座

31. Generation of direct repeats同向重复序列的产生

34. Now showingReplicative transposition File: biophoto7 \Introduction to Genetic Analysis \ Replicative transposition. (5’)

37. 10.2.2 Retrotransposons反转录转座子 LTR-RetrotransposonsLTR反转录转座子 Non-LTR Retrotransposons非LTR反转录转座子

38. LTR-RetrotransposonsLTR反转录转座子

39. Non-LTR retrotransposons非LTR反转录转座子 Weaver’s Molecular Biology, 3rd Edition, Figure 23.31

40. 10.2.3 Bacteriophage λ integrationλ噬菌体的整合 Lysogeny Lysis

41. Phage integration and excision attP: attachment sites of phage attB: attachment sites of bacterium