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Sample matrix

General Q : Should time estimates be included? E.g. Expected digestion day, Expected labeling day, Expected MS day. Q: Should it only be possible to submit ONE type of analysis per request ? Do not think so. Too tricky…too many options will be needed!. Person in charge Type of chromatography.

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Sample matrix

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  1. General Q: Should time estimates be included? E.g. Expected digestion day, Expected labeling day, Expected MS day Q: Should it only be possible to submit ONE type of analysis per request? Do not think so. Too tricky…too many options will be needed! Person in charge Type of chromatography Receiver of sample. Storage position. Person in charge Prep. of sample prior to separation typically Person in charge Storages of fractions Person in charge Data analysis Sample matrix If applicable Person in charge Data analysis Receiver of sample. Storage position. Person in charge Prep. of sample prior to digestion. Person in charge Storages of bands. Person in charge Exc. of bands Sample type: [protein] [peptide] [other -> describe] Request type Pre-MS MS Identification of proteins and peptides by mass spectrometry (bottom-up approach) Identification and quantitation of proteins and peptides by mass spectrometry (bottom-up approach) PTM enrichment (peptides) MW determination by mass spectrometry MRM validation MRM quantitation Depletion of serum or similar for abundant human proteins Person in charge Digestion: FASPin-gelin-solutionMulti-digestion (e.g. LysC+tryp) Person in charge Post-digestion,pre--MS handling Person in charge LC-MS Person in charge Data analysis Receiver of sample. Storage position. Person in charge Prep. of sample prior to digestion. Is such a box possible? Person in charge Digestion: FASPin-gelin-solutionMulti-digestion (e.g. LysC+tryp) Person in charge Data analysis Post-labeling, Pre-MS handling Person in charge Post-digestion handling Person in charge iTRAQ-type + dimethy: Labeling Person in charge LC-MS Receiver of sample. Storage position. Person in charge Prep. of sample prior to digestion. AQUA: info re. spiked in peptide(s) <- Seq + amunts SILAC: info re. used amino acids Report? Report Report Report Report Report Report Receiver of sample. Storage position. Person in charge Prep. of sample prior to MS Person in charge MS Person in charge Data analysis If know, what is expected MW If know, what is expected purity Drop-down with percentages Post-enrichment, Pre-MS handling Person in charge. Enrichment Receiver of sample. Storage position. Person in charge Prep. of sample prior to enrichment Person in charge LC-MS Person in charge Data analysis Person in charge Depletion Person in charge Storages of depleted fraction(s) Receiver of sample. Storage position. Person in charge Prep. of sample prior to digestion. Depletion of abundant human proteins from serum or similar Simple [ ] 6 most abundant [ ]

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