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DIGESTING LACTOSE WITH LACTASE

DIGESTING LACTOSE WITH LACTASE. Lab Conclusions. Background Information. Part A Directions. Fill each clean test tube with 1 mL of the sample to be tested.

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DIGESTING LACTOSE WITH LACTASE

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  1. DIGESTING LACTOSE WITH LACTASE Lab Conclusions

  2. Background Information

  3. Part A Directions • Fill each clean test tube with 1 mL of the sample to be tested. Test tube 1: 1ml glucose solutionTest tube 2: 1ml milkTest tube 3: 1ml lactase solutionTest tube 4: 1ml milk plus 3 drops lactase solution (swirl gently to mix)

  4. Part B Directions • Perform a Glucose test on each test tube using Benedict ’s reagent • Add 5-7 drops of Benedict’s Solution. • Heat test tube in a hot water bath for 3-5 minutes. • Check/Record color change. • A positive result will be orange.

  5. TABLE 1: RESULTS OF TEST FOR GLUCOSE

  6. Conclusions • Lactose in milk can be broken down into glucose and galactose. • We tested to see if the lactose in milk was broken down by detecting glucose • We used Benedicts’ reagent to detect glucose • The milk tested positive for glucose with lactase added in test tube 4 but was negative for glucose with out the lactase in test tube 2 • We can conclude that the added lactase caused lactose to be broken down because of the positive glucose test

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