'Differential expression' diaporamas de présentation

Gene Expression Analysis

Gene Expression Analysis. Benny Shomer January 2006. Whole in situ hybridization with the myeloid-specific marker, lysozyme C. NOTE!!! Studying a static RNA expression level is far from providing the ultimate answer!.

1.37k views • 97 slides

Comparative analysis of RNA- Seq data with DESeq and DEXSeq

Comparative analysis of RNA- Seq data with DESeq and DEXSeq. Simon Anders EMBL Heidelberg. Two applications of RNA- Seq. Discovery find new transcripts find transcript boundaries find splice junctions

1.9k views • 93 slides

1.09k views • 71 slides

Alexandros Kanterakis 17-5-2005 Heraklion Crete

Alexandros Kanterakis 17-5-2005 Heraklion Crete. Presentation Outline. DNA and Microarray Experiments From Genomic to Post-Genomic Informatics Combined Clinico-Genomic Knowledge Discovery Towards Reliable Gene-Markers: Supervised Gene Selection

939 views • 61 slides

Brief workflow

1.01k views • 59 slides

An Overview of Weighted Gene Co-Expression Network Analysis

An Overview of Weighted Gene Co-Expression Network Analysis. Steve Horvath University of California, Los Angeles. Contents. How to construct a weighted gene co-expression network? Why use soft thresholding? How to detect network modules? How to relate modules to an external clinical trait?

727 views • 51 slides

Preprocessing and normalization of microarray data, cell-based assays, mass spectrometry

Preprocessing and normalization of microarray data, cell-based assays, mass spectrometry Uni- and multivariate statistical analysis methods Machine Learning Harvesting and using metadata from biological databases Visualization Graphs and Networks in molecular biology

649 views • 37 slides

Detecting Differentially Expressed Genes

Detecting Differentially Expressed Genes. Pengyu Hong 09/13/2005. Background (Microarray). Extract RNA. Cells. Background. Extract RNA. Cells. Background. Extract RNA. Cells. Background. Extract RNA. Cells. Background. Extract RNA. Cells. 10 4 + genes. Background. Extract RNA.

449 views • 27 slides

BMS 617

BMS 617. Lecture 15: High-throughput sequencing and bioinformatics. High-throughput sequencing. High-throughput sequencing ( a.k.a next-generation sequencing, or NGS) is an experimental technique that can sequence large numbers of DNA fragments at one time Basic idea:

487 views • 27 slides

Statistical Analysis of Microarray Data

455 views • 26 slides

Statistical Analysis of Microarray Data

435 views • 26 slides

Exome / genome re-sequencing

Raw Illumina reads FASTQ. Exome / genome re-sequencing. Quality control (QC). Filtering. Mapping. Reference annotation. Call variants. Annotate & filter variants. Raw Illumina reads FASTQ. Quality control (QC). Filtering. Mapping. Call variants. Annotate & filter variants.

429 views • 25 slides

Presentation By: Erica Green Dr. Ely Biology 303 August 25 th , 2011

312 views • 19 slides

The Evolution of Hibernation in Mammals

Keri Massie and Emily Williamson Biology 425, Spring 2009. The Evolution of Hibernation in Mammals. Introduction. many theories about the evolution of hibernation (Kemp, 2005). None have extra-ordinary evidence supporting them (Kemp, 2005).

522 views • 17 slides

Tag profiling is dead...

Tag profiling is dead... . ...long live mRNA- Seq !. Claudia Voelckel Patrick Biggs. October 2009. Expression Studies in the New Zealand Flora. Hybridization & polyploidy. Ourisia. Ranunculus. Hebe. Species diversification & local adaptation. Pachycladon. Nothofagus. Totara.

272 views • 15 slides

Biases in RNA- Seq data

Biases in RNA- Seq data . Transcript length bias. Two transcripts of length 50 and 100 have the same abundance in a control sample. The expression of both transcripts is doubled in a treatment sample. The biological variance is the same for both transcripts.

272 views • 15 slides

PD Evaluations and Pathway Analysis of 2 PARP Inhibitors: ABT-888 vs. BSI201

PD Evaluations and Pathway Analysis of 2 PARP Inhibitors: ABT-888 vs. BSI201. J Ji & M Lee 11-12-2010. Two PARP Inhibitors: ABT-888 vs. BSI201. Lab Questions. Is there difference in PD response? Does agent induce g h2AX?

363 views • 14 slides

Significance analysis of microarrays (SAM)

Significance analysis of microarrays (SAM). SAM can be used to pick out significant genes based on differential expression between sets of samples. Currently implemented for the following designs: - two-class unpaired two-class paired multi-class censored survival one-class. SAM.

333 views • 9 slides

Next-generation sequencing and PBRC

Next-generation sequencing and PBRC. Next Generation Sequencer Applications. DeNovo Sequencing Resequencing, Comparative Genomics Global SNP Analysis Gene Expression Analysis Methylation Studies ChIP Sequencing-transcription factors, histones, polymerases

300 views • 8 slides

Table 2: Genes expressed only in 0 m M Al and also expressed in the YEPD (yellow array)

Investigating effects of aluminum on Saccharomyces cerevisiae : A model for laboratory-based investigative teaching Maria Farrell and Emily Schmitt Nova Southeastern University Department of Math, Science, and Technology Ft. Lauderdale, FL. DISCUSSION. INTRODUCTION.

113 views • 1 slides