'Gfp gene' diaporamas de présentation

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Lesson Overview

Lesson Overview. 15.1 Selective Breeding. Show short videos from Nova. REVIEW. What are the two things that can get mutated (when talking DNA)? Yes! Genes & Chromosomes Did you know that some people make MUTATIONS on purpose?. THINK ABOUT IT. Where does popcorn come from?

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Bacterial Transformation

Bacterial Transformation. BRIDGES 2014. Central Dogma. DNA?RNA?PROTEIN?TRAIT. Phenotype. Genotype. Transformation. Adds new information to DNA Cell can take up ( take inside) and expresses a new piece of DNA Transformation

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Bacterial Transformation and GFP stories

Bacterial Transformation and GFP stories. Kate Andrews Lorraine Bruce Marjorie Smith. The bacterium. Bacterial chromosome. Plasmids. Cytoplasm. Ribosomes. Cell wall. Cell membranes. The plasmid. Origin of replication.

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Genetic Transformation

Genetic Transformation. WHY SHOULD WE DO THIS?. Genetic Engineering is now widely used:. Bacteria that produce human insulin. Corn that produces insecticide. Rice that produces extra vitamin A. Goats that produce spider silk. IMPORTANT TERMS. Promoter - Plasmid - Transformation

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GFP Transformation Lab

GFP Transformation Lab. Images taken without permission from http://upload.wikimedia.org/wikipedia/de/4/4d/Protein_GFP_1EMA.png , http://bioinfo.biotec.or.th/Picture/Cell%20Tutorial/image005.jpg , http://www.plantsci.cam.ac.uk/Haseloff/SITEGRAPHICS/Jellyfish.jpeg.

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Modeling Transformation

Modeling Transformation. What does each step do?. Transformation Procedure. Transformation Procedure. Building Your Model. Yarn = chromosomal DNA Beads = ribosomes Black velcro = plasmid DNA Green velcro = GFP gene Pink velcro = Ampicillin resistance gene Bottle = cell membrane

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GFP Transformation Lab

GFP Transformation Lab. GFP Transformation Lab. Images taken without permission from http://upload.wikimedia.org/wikipedia/de/4/4d/Protein_GFP_1EMA.png , http://bioinfo.biotec.or.th/Picture/Cell%20Tutorial/image005.jpg , http://www.plantsci.cam.ac.uk/Haseloff/SITEGRAPHICS/Jellyfish.jpeg.

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Make a new entry- Entry 49:pGLO Quiz Review -5/9/12 See attached worksheet

Make a new entry- Entry 49:pGLO Quiz Review -5/9/12 See attached worksheet. 1. What are plasmids? What type of organism generally has plasmids Plasmids are circular, double stranded DNA found in bacteria. Bacterium. 2.Describe the function of a. a restriction enzyme -

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AP Bio: Wednesday, 2.16.11 Planning Lab 6A; Mutations

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Make a new entry- Entry 49:pGLO Quiz Review -5/9/12 See attached worksheet

Make a new entry- Entry 49:pGLO Quiz Review -5/9/12 See attached worksheet. 1. What are plasmids? What type of organism generally has plasmids Plasmids are circular, double stranded DNA found in bacteria. Bacterium. 2.Describe the function of a. a restriction enzyme -

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GFP Transformation Lab

GFP Transformation Lab. Images taken without permission from http://upload.wikimedia.org/wikipedia/de/4/4d/Protein_GFP_1EMA.png , http://bioinfo.biotec.or.th/Picture/Cell%20Tutorial/image005.jpg , http://www.plantsci.cam.ac.uk/Haseloff/SITEGRAPHICS/Jellyfish.jpeg.

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Green Fluorescent protein

Green Fluorescent protein. Samantha Stickdorn. Discovery. Discovered by Osamu Shimoura in 1962 First discovered in Aequorea Victoria a hydrozoan jellyfish species he extracted the protein from a faintly luminescent liquid called squeezate

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Summer 2008 Workshop in Biology and Multimedia for High School Teachers

Summer 2008 Workshop in Biology and Multimedia for High School Teachers. AP Biology Lab 6: Genetic Engineering via Bacterial Transformation. Making E. coli glow like jellyfish. Amy Dickson, Prospect Hill Academy Charter School All images by Christine Rodriguez and Amy Dickson.

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Summer 2008 Workshop in Biology and Multimedia for High School Teachers

Summer 2008 Workshop in Biology and Multimedia for High School Teachers. AP Biology Lab 6: Genetic Engineering via Bacterial Transformation. Making E. coli glow like jellyfish. Amy Dickson, Prospect Hill Academy Charter School All images by Christine Rodriguez and Amy Dickson.

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pGLO Transformation Lab

pGLO Transformation Lab. Many organisms can glow!. Jellyfish. Amphipods Oceanexplorer.noaas.gov/explorations/05deescope/background/fluorescence. www.worldnetcams.com/sealife/cerianthus.jpg. Corals. Copepods Oceanexplorer.noaas.gov/explorations/05deescope/background/fluorescence.

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Genetic Transformation of Bacteria and Gene Regulation

Genetic Transformation of Bacteria and Gene Regulation. Transform E.coli cells with plasmid The plasmid has ampicillin resistant gene. Green fluorescent protein gene Natural source of this gene jelly fish Aequorea victoria

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Human cells stained with an antibody for the Tubulin protein Image from Molecular Probes

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Bernard et al, Supplementary Figure S4

Bernard et al, Supplementary Figure S4. pTRE. pTRIP-DU3-TRE-MCS. Supplementary Figure S4. The pTRIP-DU3-TRE-MCS lentivector.

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Fluorescence of GFP chromophore by cyclization reaction including rearrangement and oxidation

Green fluorescent protein ( GFP ). composed of 238 amino acids (26.9 kDa ), originally isolated from the jellyfish Aequorea victoria fluoresces green when exposed to blue light Used as a reporter of expression & biosensor The GFP gene can be introduced into

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