'Template dna' diaporamas de présentation

Section G RNA synthesis and processing

Section G RNA synthesis and processing. Transcription in prokaryotes Transcription in eukaryotes: an overview Transcription in eukaryotes Processing of eukaryotic pre-mRNA Ribosomal RNA Transfer RNA. RNA Transcription in Prokaryotes.

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Chapter7 Analyzing DNA ? gene structure, variation ? expression

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biotechnology

biotechnology. Cloning. Recap What is cloning? Generating identical copies of organisms, cells, or replicating nucleic acid sequences from organisms Dolly (the sheep) is a clone, but a natural identical twin is not a clone. DNA sequence amplified by growth is a clone,

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1.08k views • 38 slides

How PCR Works

How PCR Works. How PCR works. Bio-Rad Animation Cold Spring Harbor Animation Animated .GIF files. Review: The structure of DNA. Double Helix. Complementary Base Pairing. How PCR works. Cold Spring Harbor Animation. How PCR works. Bio-Rad PCR Animation. How PCR works.

471 views • 31 slides

REPLICATION

REPLICATION. Chapter 7. The Problem. DNA is maintained in a compressed, supercoiled state. BUT, basis of replication is the formation of strands based on specific bases pairing with their complementary bases. ? Before DNA can be replicated it must be made accessible, i.e., it must be unwound.

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PV92 PCR

PV92 PCR. Alu insert, PV92 locus, chromosome 16. What can you do with the Chromosome 16 PV92 PCR kit ?. Introduce the polymerase chain reaction (PCR) technique Apply PCR to population genetics Directly measure human diversity at the molecular level Compare results to published data online.

1.41k views • 29 slides

Chapter 3: DNA Replication Models of DNA replication: Meselson-Stahl Experiment DNA synthesis and elongation DNA polyme

Chapter 3: DNA Replication Models of DNA replication: Meselson-Stahl Experiment DNA synthesis and elongation DNA polymerases Origin and initiation of DNA replication Prokaryote/eukaryote models (circular/linear chromosomes) Telomere replication

2.05k views • 28 slides

Biotechnology in the NSS biology curriculum

Biotechnology in the NSS biology curriculum. Daniel K. L. Lee, Ph. D. Department of Applied Biology and Chemical Technology The Hong Kong Polytechnic University. Rapidly expanding area Understand the principles of the techniques Familiarize oneself with the applications

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Biotechnology in the NSS biology curriculum

Biotechnology in the NSS biology curriculum. Daniel K. L. Lee, Ph. D. Department of Applied Biology and Chemical Technology The Hong Kong Polytechnic University. Rapidly expanding area Understand the principles of the techniques Familiarize oneself with the applications

408 views • 27 slides

Developing multiplex PCR assays for human identity testing - is there overlap with pathogen screening?

Developing multiplex PCR assays for human identity testing - is there overlap with pathogen screening?. Dr. Peter M Vallone Human Identification Project U.S. National Institute of Standards and Technology SoGAT XX Warsaw, Poland 12-13 June 2007. Human Identity Project at NIST.

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Restriction Digestion and Analysis of Lambda DNA Kit

Restriction Digestion and Analysis of Lambda DNA Kit. What can you do with the Restriction Digestion and Analysis of Lambda DNA Kit?. Understand the use of restriction enzymes as biotechnology tools Become familiar with principals and techniques of agarose gel electrophoresis

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Basic Molecular Biology Information

Basic Molecular Biology Information. Central Dogma of Molecular Biology. Concerns the flow of information in the cell. Nucleic Acids. Only 2 types: DNA and RNA linear chains of nucleotides DNA: 2 chains running anti-parallel twisted together into a double helix

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Polymerase Chain Reaction

PCR. Polymerase Chain Reaction. PCR. Is a revolutionary method developed by Kary Mullis in1983. PCR is used in molecular biology to make many copies of small sections of DNA.

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How do we analyze DNA?

How do we analyze DNA?. Gel electrophoresis Restriction digestion Sequencing. DNA analysis. How do we manipulate DNA to improve our crop? First we need to identify which genes in the DNA sequence are important for the trait we are trying to improve

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The Polymerase Chain Reaction

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LAB 5: IDENTIFICATION OF PATHOGENIC BACTERIA BY PCR AMPLIFICATION OF THE 16s rRNA GENE & RESTRICTION FRAGMENT

LAB 5: IDENTIFICATION OF PATHOGENIC BACTERIA BY PCR AMPLIFICATION OF THE 16s rRNA GENE & RESTRICTION FRAGMENT LENGTH POLYMORPHISM (RFLP). OBJECTIVES. Describe process & principles of PCR technique Describe how the 16 S rRNA can be used in bacterial identification

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SOUTHERN BLOT Capillary Transfer of DNA to a Membrane

SOUTHERN BLOT Capillary Transfer of DNA to a Membrane. ABE WORKSHOP JUNE 6-24, 2005. Purpose. Detection of specific sequences among DNA fragments separated by gel electrophoresis. Restriction fragments appear as a smear rather than as discrete bands on the gel.

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Polymerase Chain Reaction

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Gopher Tortoise Genetics from Moody Air Force Base, Georgia David Mederos, Department of Biology

Gopher Tortoise Genetics from Moody Air Force Base, Georgia David Mederos, Department of Biology Faculty Sponsors: Dr. J. Mitchell Lockhart and Dr. John F. Elder, Department of Biology Co-Author: Gregory Lee, Moody Air Force Base.

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