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Gene Expression: From Gene to Protein

Gene Expression: From Gene to Protein. 0. 14. Questions prepared by Brad Stith, University of Colorado Denver Louise Paquin, McDaniel College. The final product of an expressed gene can be which of the following? Also note the function of each. mRNA tRNA rRNA polypeptide.

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Gene Expression: From Gene to Protein

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  1. Gene Expression: From Gene to Protein 0 14 Questions prepared by Brad Stith,University of Colorado Denver Louise Paquin,McDaniel College

  2. The final product of an expressed gene can be which of the following? Also note the function of each. • mRNA • tRNA • rRNA • polypeptide

  3. The final product of an expressed gene can be which of the following? Also note the function of each. mRNA tRNA rRNA polypeptide

  4. Of the following, which pertain to replication, transcription, modification, or translation? Each can be used once or more than once.

  5. The template strand of a given gene includes the sequence 3-G C C A C G T A T C A G-5 A. What is the sequence of the nontemplate strand? B. What is the mRNA sequence made? For each one, be sure to indicate 5 and 3 ends.

  6. Which of the following is the best example of gene expression? A frog adapts to variation in its environmental temperature. Mouse fur color results from pigment formed by gene-encoded enzymes. DNA is replicated during the S phase of the cell cycle. The percent of A versus a alleles in a population is altered by natural selection. Mutation alters the sequence of a region of DNA.

  7. Which of the following is the best example of gene expression? A frog adapts to variation in its environmental temperature. Mouse fur color results from pigment formed by gene-encoded enzymes. DNA is replicated during the S phase of the cell cycle. The percent of A versus a alleles in a population is altered by natural selection. Mutation alters the sequence of a region of DNA.

  8. Give some examples that demonstrate the ability of genes from one species to be expressed in a different species. This is possible because of which property of the genetic code?

  9. Describe what is occurring at each of the stages of transcription (1, 2, and 3 in the figure). How does each stage move the process forward?

  10. What components form the transcription complex at a eukaryotic promoter?

  11. How and where are modifications made to eukaryotic mRNA before it goes to the cytosol?

  12. What is represented at each of the lettered sites, and what occurs at each of these sites during translation?

  13. On this model of a tRNA molecule, identify each of the following: 5 and 3 ends hydrogen bonds amino acid attachment site anticodon loop

  14. Aminoacyl-tRNA synthetase is an enzyme whose function is to ________. To do so, it binds a molecule of amino acid, and two other molecules: ________ and ________. After dephosphorylation, which two molecules are joined by a covalent bond? For the appropriate tRNA to be joined to the amino acid, what must occur?

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