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4. InSight DeepSee : new ultrafast lasersystem

Tunable ultrafast oscillators. 4. InSight DeepSee : new ultrafast lasersystem Broadest tuning range for deepest imaging 680 – 1300 nm Average output power: 0,6 – 1,3 W Pulse width: < 120 fs Repetation rate: 80 MHz Complete automatized system with integrated DeepSee

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4. InSight DeepSee : new ultrafast lasersystem

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  1. Tunable ultrafast oscillators 4. InSight DeepSee: new ultrafast lasersystem Broadest tuning range for deepest imaging 680 – 1300 nm Average output power: 0,6 – 1,3 W Pulse width: < 120 fs Repetation rate: 80 MHz Complete automatized system with integrated DeepSee Dual wavelength option for uncaging, CARS, and multi-modal imaging

  2. Short Pulse Width Across Tuning Range Short Pulses Across 600nm Tuning Range Delivered to the Sample

  3. Lower average power at the peak. Higher average power at higher wavelengths

  4. Average power comparision of Insight and OPO

  5. Peak power comparision of Insight and OPO

  6. Layouts for Longer Wavelengths Simplified beam routing means: • Less equipment (AOMs) needed • Simplified user interface - Less space is necessary • Easier access to full tuning range

  7. Dual Wavelength Output Option for Multi-Modal Imaging • 1,041 nm fixed wavelength 500 mW output • Synchronized to tunable output with fixed delay • Enables dual-wavelength imaging, uncaging and CARS

  8. Applications Biological imaging techniques: • Two photon excited florescence (TPEF) imaging • SHG imaging • THG imaging • Multi-modal imaging • Femtosecond CARS • Autofluorescence • Two-photon uncaging Other Applications: • Time-resolved photoluminescence • Non-linear spectroscopy • Optical computed tomography • Terahertz imaging • Semiconductor metrology

  9. Images for Neuroscience Studies a) b) c) Sample: Mouse Barrel Cortex Red: Texas Red (1050nm) Sample: hippocampus CA3 pyramidal neuron Red: Alexa 594 (830nm) Sample : H-line mouse eYFP by Thy-1 Promoter(1030nm) References: a) Department of Molecular Medicine for Pathogenesis, Ehime University Graduate School of Medicine, Prof. Naoki Honkura and Prof. Takeshi Imamura b) University of Melbourne, Australia, Gabriel Jones c) Dominick P. Purpura Department of Neuroscience, Albert Einstein College of Medicine, Karina Alviña, Ph.D.

  10. Marine Biology a) a) Sample: sea urchin H2B-mCherry (980nm) eGFP (980nm) Sample: zebra fish H2B-Venus (1150nm) E2-Crimson (1150nm) Reference: a) Institut de Neurobiologie, Alfred Fessard, Gif Sur Yvette, France, Prof Nadine Peyrieras

  11. Images for Nutritional Studies a) b) Sample: C. elegans Blue: CARS (798nm+1040nm) Green: GFP (920nm) Red: dsRed (1040nm) Sample: Fatty Liver Orange: SRS (802nm+1040nm) References: a) University of California, Irvine, Prof Eric Potma b) Purdue University , Prof Ji-Xin Cheng

  12. Images for Cancer Studies a) b) c) Sample: Human Eyelid Red: CARS (798nm+1040nm) Blue: SHG (798nm) Sample: Human Dermal Tissue Green: SHG (800nm) Red: THG (1150nm) Sample: Lymph Node Green: GFP (920nm) Red: Alexa 546 (1041nm) References: a) University of California, Irvine, Prof Eric Potma b) University of California, Irvine, Prof Eric Potma c) Monash Institute of Pharmaceutical Sciences, Monash University, Cameron J. Nowell

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