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a. DNA ligase b. DNA polymerase c. RNA polymerase d. Restriction enzyme

a. DNA ligase b. DNA polymerase c. RNA polymerase d. Restriction enzyme e. Reverse transcriptase f. Transformation 1. Enzyme found in retroviruses that produce DNA from an RNA template. 2. Enzyme used during replication to attach Okazaki fragments to each other.

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a. DNA ligase b. DNA polymerase c. RNA polymerase d. Restriction enzyme

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  1. a. DNA ligase b. DNA polymerase c. RNA polymerase d. Restriction enzyme e. Reverse transcriptase f. Transformation 1. Enzyme found in retroviruses that produce DNA from an RNA template. 2. Enzyme used during replication to attach Okazaki fragments to each other. 3. Our bacteria that produced biolumescent proteins. Answer: e Answer: a Answer: f

  2. Put the following phrases in order to form a • plasmid carrying recombinant DNA. • 1. use restriction enzyme • 2. use DNA ligase • 3. remove plasmid from parent bacterium • 4. introduce plasmid into new host bacterium • 1, 2, 3, 4 • 4, 3, 2, 1 • 3, 1, 2, 4 • 2, 3, 1, 4 Answer: c

  3. Restriction endonucleases (enzymes) • cut DNA. • are naturally found in prokaryotic cells. • are naturally found in eukaryotic cells. • both a and b • all of the above Answer: d

  4. The separation of DNA fragments by gel • electrophoresis is primarily achieved by • differential • sizes of fragments. • charges of fragments. • solubilities of fragments. • cleavage points of fragments. • radioactivity of fragments. Answer: a

  5. Which of the following is used for amplification? • Transformation • Polymerase chain reaction (PCR) • Restriction fragment length polymorphism • (RFLP) • d. Recombinant DNA technology • e. All of these Answer: b

  6. Which of the following is NOT needed to • make a recombinant DNA molecule? • foreign DNA • vector DNA • restriction enzymes • DNA ligase • DNA polymerase Answer: e

  7. Genetically engineered plants have or will • be used to • resist insects • resist herbicides • produce protein-enhanced beans, corn, and • wheat • d. produce animal neuropeptides, blood factors, • and growth hormones. • e. All of these are correct. Answer: e

  8. DNA that is made from mRNA is called • nonsense DNA (nDNA) • antisense DNA (aDNA) • complementary DNA (cDNA) • uncomplementary DNA (uDNA) • recombinant DNA (rDNA) Answer: c

  9. Foreign DNA can be inserted into vector DNA • because both DNA molecules • have the same genes. • have the same bases. • have “sticky ends”. • are not complementary to each other. • All of these are correct. Answer: c

  10. Which enzyme is used to seal breaks in a • DNA molecule? • DNA polymerase • RNA polymerase • restriction enzymes • DNA ligase • RNA ligase Answer: d

  11. Answer: E • Blastula stage • Beginning of gastrulation • Zygote • Formation of germ layers Answer: F Answer: A Answer: G

  12. Lane with smallest • band • 2. Lane showing most • restriction sites • 3. Lane with DNA • most closely related • to that in lane F Answer: A Answer: A Answer: B

  13. Which of the following describes the correct • sequence of stages during embryogenesis? • cleavage, blastula formation, gastrulation • cleavage, gastrulation, blastula formation • blastula formation, gastrulation, cleavage • blastula formation, cleavage, gastrulation • gastrulation, cleavage, blastula formation Answer: A

  14. In animals, all of the following are associated • with embryonic development EXCEPT • migration of cells to specific areas. • formation of germ layers. • activation of all the genes in each cell. • inductive tissue interactions. • cell division at a relatively rapid rate. Answer: C

  15. Cell differentiation • results from the loss of particular genes • from the nucleus of the differentiated cell. • b. results from the differential expression • of genes that are responsive to environmental • signals. • c. involves the persisting totipotency of early • embryonic cells in the mature organism. • d. results from the mutations in genes that • control the synthesis of DNA. • e. precedes cell determination. Answer: B

  16. Gel electrophoresis can be used for which of • the following purposes? • To group molecules based on their polarity • To measure the acidity of certain large • molecules • To measure the polarity of certain large • molecules • To separate out the proteins in a mixture • To measure the amount of protein in a • mixture of substances Answer: D

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