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SNAPs and SNAREs

Membrane system- diverse; Transport vesicle system- Selective in nature<br>Transport vesicles : surface markers ( depending on origin & cargo type) that recognizes complementary receptors (Target membrane) <br>2 protein class in control- 1. SNARES (20 types) 2. Rabs ( GTPases targeting) <br>SNARE proteins -central role; i) specificity, ii) bringing the fusion (vesicles with the membrane in target.) Endocytic routes.<br>Exist in supportive sets- 1. v-SNAREs i.e vesicle membrane SNAREs <br> 2. t-SNAREs i.e target membrane SNAREs.

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SNAPs and SNAREs

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  1. PRESENTATION BY: ARCHITA KEDIA M.Sc LIFE SCIENCES (BIOTECH.) PART II SEMESTER IV PAPER- IV UNIVERSITY OF MUMBAI SNAPs AND SNAREs

  2. MEMBRANE TRANSPORT via SNARE protein and GTPases (Targetting) • Membrane system- diverse; Transport vesicle system- Selective in nature • Transport vesicles : surface markers ( depending on origin & cargo type) that recognizes complementary receptors (Target membrane) • 2 protein class in control- 1. SNARES (20 types) 2. Rabs ( GTPases targeting) • SNARE proteins -central role; i) specificity, ii) bringing the fusion (vesicles with the membrane in target.) Endocytic routes. • Exist in supportive sets- 1. v-SNAREs i.e vesicle membrane SNAREs • 2. t-SNAREs i.e target membrane SNAREs. • T-SNARE (trans), clasp 2 membranes together.

  3. STRUCTURE OF SNARE SNARE motif (cytosolic domain of SNARE segment) comprised of ; • Heptadrepeats (coiled- structures); 60-70 amino acids. • Tightly reversible assembly by V- and t-SNAREs – c/a "trans"-SNARE complexes (formed of 4 -helix bundles). • 3 SNAREs (In synaptic vesicles) : • 1. syntaxin 1 (one helix) • 2. SNAP-25 (2 helix) • 3. Synaptobrevin (VAMP) - ( 1 helix)

  4. SNAREs that're interacting, are separated apart before they can re-enact again through the action of NSF. • Rab proteins - facilitate vesicle docking rate & its regulation, matching of v-SNAREs and t-SNAREs

  5. SNARE HYPOTHESIS • (v-SNAREs) SNARE Vesicle-SNAP-receptors • (t-SNAREs) -Target-SNAP-receptors • SNAP=soluble NSF attachment proteins • NSF=N-ethylmaleimide-sensitive factor

  6. SNAP ( SOLUBLE NSF ATTACHTMENT PROTEINS) • Cytosolic adaptor proteins • Intricated in intracellular transport during membrane vesicular fusion &exocytosis. • Event of vesicle fusion- Assembly and de-assembly. • SNAP + SNARE + NSF = 20S complex. • SNAPs receptor proteins- syntaxin 1, • SNAP-25 (synaptosome associated protein, 25kDa), • and VAMP (synaptobrevin). • Toxicological Significance - blocks transmission- 20S- target neurotoxins, Botulinum

  7. Human SNAP of yeast homologue (Sec17), depicting assembly and disassembly in vesicle fusion membranes with 20 S complex

  8. REFERENCES • Alberts, B., & Wilson, J. (2008). Molecular biology of the cell. Taylor and Francis, Garland. • Snare (protein). Wikiwand. (n.d.). • Wikimedia Foundation. (2022, January 25). Soluble NSF attachment protein. Wikipedia.

  9. THANK YOU FOR LISTENING!

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