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Use IPTG and DTT for Experimental Purposes

IPTG | 367-93-1 | Isopropyl-Beta-D-Thiogalactoside |Jiwan is a compound that is used in the procedure of cloning. It is used with bacterial colonies especially Escherichia coli. The compound is used with beta-galactosidase.

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Use IPTG and DTT for Experimental Purposes

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  1. Use IPTG and DTT for Experimental Purposes IPTG | 367-93-1 | Isopropyl-Beta-D-Thiogalactoside |Jiwan is a compound that is used in the procedure of cloning. It is used with bacterial colonies especially Escherichia coli. The compound is used with beta-galactosidase. The compound is used only for research in life science. There is no use of this compound in diagnosis purposes. IPTG is a molecular mimic of lactose metabolite and its presence activates lac operon for gene transcription. The atom of sulfur present in this compound prevents the cells from degrading the inducer. Test Report: PH Value: 5.86 Clarity: colorless clear absorbance: A330nm: 0.073; A400nm: 0.038 Specific rotation: -29.6 degrees Water: 0.8% Melting point: 118.7-123.7 Purity: 99.5%        Usage: IPTG is β galactosidase activity inducing substance. Based on this feature, when a PUC series vector DNA (or other vector with a lacZ gene DNA) deletion in lacZ host cells are transformed with the vector during DNA M13 phage were transfected or, if added to the medium in the plate X -gal and IPTG, sinceβ- galactosidase complementarity of α-, may appear white depending on whether colonies (or plaques) and easily pick out recombinants.

  2. In addition, it can be used as having a lac or tac promoter expression vectors and other expression inducer. Now more and more areas have been used IPTG, undergoing tremendous role in the field of production, pharmaceutical, biotechnology, etc. for companies to bring greater efficiency, improve product quality and shorten the reaction time. DTT | CAS# 3483-12-3 or Dithiothreitol is a compound that is used in biochemical studies. The compound is used to prevent the oxidation of SH groups. The main task of this compound is to break the bonds in protein disulfide. The compound also helps in stabilizing enzymes and other proteins. The compound is manufactured by Jiwan Pharmtech, a Chinese company. In order to break the bond in protein disulfide, two sequential reactions are conducted which results in the oxidation of the DTT molecule and reduces the disulfide bond. The power of this molecule reduces if the pH value of the compound in which it is used is greater than 7. IPTG and DTT are used only for experimental purposes. Address Contact us Jiwan Pharmaceutical Technology Co., Ltd. Street:- No. 1222 West Aoti Road Ph- 86-53155519016 City :- jinan Zip or Postal Code :- 250000 visit here State :- Shandong http://jiwanchem.com

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