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DNA sequencing

DNA sequencing. Technique used to identify sequence of bases The nucleotides are separated from each other in the order that they are found in strand of DNA. Nucleotides appear as dark bands The sequence in the first segment of DNA reads CTGA, second segment TACG

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DNA sequencing

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  1. DNA sequencing • Technique used to identify sequence of bases • The nucleotides are separated from each other in • the order that they are found in strand of DNA. • Nucleotides appear as dark bands • The sequence in the first segment • of DNA reads CTGA, • second segment TACG • This is how DNA fingerprint is • compiled - Unique for every person C T A G

  2. DNA Fingerprinting • DNA fingerprinting is often used by the police in • identifying the suspect of a crime. • A useful but controversial method • A sample of a suspect’s bodily fluid or tissue is to be • compared with a sample found at the scene of a • crime. • The pattern of lines represents a person’s specific • genetic make-up. • DNA fingerprinting use in 9/11 disaster to identify victims

  3. Assessment of DNA fingerprinting LO2 • Read the following: Inspector Ndlovu were investigating the scene of a violent crime. The victim was a 70 –year old woman. She have been stabbed and left to die. They found a view pieces of hair in one of her hands. There was also skin under her nails. They arrested three possible suspects. DNA samples were taken from all three and the victim. DNA fingerprint samples of the four samples was compared with the DNA fingerprints taken from the crime scene.

  4. DNA sample from hair Victim Suspect 1 Suspect 2 Suspect 3 Identify the suspect responsible for the crime out of above evidence

  5. Ethics of DNA Fingerprinting • Discuss the misuse of DNA fingerprinting by using examples: • DNA fingerprint results may be used for unauthorized purposes such as to identify individuals with certain stigmatizing illnesses. • The potential for misuse increases if DNA fingerprint results are kept in a database: like fingerprints, however, the police are obliged to destroy DNA evidence if a defendant is acquitted. • There is also concern that insurance companies will screen potential policyholders to exclude those carrying genetic disorders.

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