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Gene Expression Analysis in Mycorrhizal-Potato Cultures

RNA extracted from potato cultures colonized with F. mosseae, A. euteiches, or both fungi was analyzed by real-time RT-PCR. Mean values and standard deviations were compared to controls, normalized by M. truncatula TEF1 gene expression.

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Gene Expression Analysis in Mycorrhizal-Potato Cultures

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  1. PAL CHS CHR IFR COMT Fig S2 Gene expression analysis in pot cultures. RNA was extracted from pot cultures and transcript accumulation was analysed by real-time RT-PCR. Shown are mean values and standard deviations obtained with RNA from roots either colonised with F. mosseae (F) with A. euteiches (A) or with both fungi (FA) compared to controls (C). All RNA accumulation values were normalized by the expression of the M. truncatula TEF1 gene. If significant interactions between the factors mycorrhization and pathogen were detected (two-way ANOVA; P = 0.05; n = 3), different letters above the columns indicate significant differences between treatments (Fisher’s LSD; P = 0.05; n = 3).

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