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MIC 303 INDUSTRIAL AND ENVIRONMENTAL MICROBIOLOGY

MIC 303 INDUSTRIAL AND ENVIRONMENTAL MICROBIOLOGY. INTRODUCTION: INDUSTRIAL MICROBIOLOGY & FERMENTATION. Mdm Aslizah Mohd Aris. Biotechnology. Use of microorganisms, cells, or cell components to make a product Classical: Fermentation Modern: Recombinant DNA.

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MIC 303 INDUSTRIAL AND ENVIRONMENTAL MICROBIOLOGY

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  1. MIC 303INDUSTRIAL AND ENVIRONMENTAL MICROBIOLOGY INTRODUCTION: INDUSTRIAL MICROBIOLOGY & FERMENTATION Mdm Aslizah Mohd Aris

  2. Biotechnology • Use of microorganisms, cells, or cell components to make a product • Classical: Fermentation • Modern: Recombinant DNA

  3. Definition – Industrial Microbiology • “Application of scientific and engineering principles to produce valuable commercial products or to carry out important chemical transformations or treatment of raw materials by means of microbiological aspects”. • This process is commonly referred to as Fermentation.

  4. RANGES OF FERMENTATION PROCESS

  5. RANGES OF FERMENTATION PROCESS

  6. Fermentation • Aerobic • Anaerobic

  7. Aerobic fermentation • Adequate aeration • Bioreactors- adequate supply of sterile air • In addition, these fermenters may have a mechanism for stirring and mixing of the medium and cells • Antibiotics, enzymes, vitamins.

  8. Anaerobic fermentation • In anaerobic fermentation, a provision for aeration is usually not needed. • Lactic acid, ethanol, wine

  9. Type of Fermentation • Submerged Fermentation • Surface Fermentation • Solid State Fermentation • Trickly Fermentation • Batch Fermentation • Continuously Stirred Tank Reactor (CSTR)

  10. Type of Fermentation • Batch Fermentation : A closed system in which the biomass (x), substrate (s) and product (P) and cell located in a closed vessel and does not leave the vessel until harvest time. • Submerged Fermentation: A fermentation process in which the microorganisms is located at the base of substrate. Usually fungal fermentation. (Liquid Fermentation). • Surface Fermentation: A fermentation process in which microorganisms / inoculums is located at the substrate surface. (Liquid Fermentation).

  11. Type of Fermentation • Solid State Fermentation: A fermentation process in which the substrate is solid. Ex: Tempe and mushroom, seed koji. • Trickly Fermentation : A process whereby the liquid substrate is trickly / drop onto wood chips previously immobilized with the inoculum. First used in traditional acetic acid fermentation.

  12. Type of Fermentation • Continuously Stirred Tank Reactor (CSTR) : A continuously stirred tank reactor or chemostat. An open fermentation system in which the biomass (x), substrate (s) and product (P) continuously leave the vessel.

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