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Biology 22

Biology 22. Drosophila Identification of various phenotypes. Outline. Brief description of Drosophila phenotypes and life cycle 1-Experiment 1: collection of data related to gender and body color 2-Experiment : 3 point Test Cross

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Biology 22

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  1. Biology 22 Drosophila Identification of various phenotypes.

  2. Outline • Brief description of Drosophila phenotypes and life cycle • 1-Experiment 1: collection of data related to gender and body color • 2-Experiment : 3 point Test Cross • How to study keep your flies still during the identification.

  3. Reference for Pictures: • Carolina Drosophila melanogster Manual (45-2620) • Published: Carolina Biological Supply Company • Based on research of Dr. Thomas Hunt Morgan

  4. 1866-1945

  5. The life Cycle

  6. Life Cycle:Drosophila melanogaster • Eggs: contain filaments • 2 molting phases “instars” • Life cycle is 10 days

  7. Fruity Fly Egg and Larva

  8. Fruit Fly Pupa and Adult

  9. 1- EXPERIMENT: Identification of: • Gender • Body Color

  10. Wild Type Versus Mutants • Body Color • Wild type: Beige • Mutant: Yellow body

  11. Parental phenotypes Experiment: body color • A= female: wt (beige) • B=female: wt (beige) • C=male: wt (beige) • D=female: mutant (yellow) • E=male: mutant (yellow)

  12. Fruit Fly Body Color

  13. Sex combs: Male Drosophila

  14. Female and Male Fruit Flies

  15. Abdominal segements • 7 segments to female abdominal segments • 5 segments to male abdominal segments • Male body size is smaller than female • But the “last” abdominal segment is larger in male

  16. Experiment 2 3 point test cross Eye color Body color Wing length 2- Experiment: 3 point test cross:

  17. 1. Body Color 2. Wing length 3. Eye Color ♦Wt vs. Mutant Beige (bl+) vs. Black (bl) Long(vg+) vs. Vestigal(vg) Brick red (br+) vs. brown (br) 3 point test cross for:

  18. Crosses: Female Males • Males and female progeny from this F2 cross are your 3 point test cross results! bl+ vg+ Bw+ bl vg bw X bl vg bw bl vg bw

  19. There are all sorts of eyes!

  20. Eyes

  21. You will have only red eyes (wt)

  22. Fruit Fly Wings

  23. Nap TimeTriethylamine

  24. How to anesthetize a fly

  25. How to use the Napper

  26. View the Fruit Fly

  27. You will record class data • After counting the crosses record the data on the class spreadsheet

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