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Identification of Drosophila Phenotypes: Gender, Body Color, and Wing Length Analysis

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This study explores the identification of various Drosophila melanogaster phenotypes, focusing on gender, body color, and wing length through carefully designed experiments. The life cycle of the fruit fly, spanning approximately 10 days, is examined, with emphasis on key distinguishing features such as body color (wild type vs. mutants) and abdominal segment counts between genders. Experimentation includes a three-point test cross to analyze eye color, body color, and wing length traits. Detailed methodologies for maintaining and observing flies are provided, ensuring accurate data collection for further study.

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Identification of Drosophila Phenotypes: Gender, Body Color, and Wing Length Analysis

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Presentation Transcript

  1. Biology 22 Drosophila Identification of various phenotypes.

  2. Outline • Brief description of Drosophila phenotypes and life cycle • 1-Experiment 1: collection of data related to gender and body color • 2-Experiment : 3 point Test Cross • How to study keep your flies still during the identification.

  3. Reference for Pictures: • Carolina Drosophila melanogster Manual (45-2620) • Published: Carolina Biological Supply Company • Based on research of Dr. Thomas Hunt Morgan

  4. 1866-1945

  5. The life Cycle

  6. Life Cycle:Drosophila melanogaster • Eggs: contain filaments • 2 molting phases “instars” • Life cycle is 10 days

  7. Fruity Fly Egg and Larva

  8. Fruit Fly Pupa and Adult

  9. 1- EXPERIMENT: Identification of: • Gender • Body Color

  10. Wild Type Versus Mutants • Body Color • Wild type: Beige • Mutant: Yellow body

  11. Parental phenotypes Experiment: body color • A= female: wt (beige) • B=female: wt (beige) • C=male: wt (beige) • D=female: mutant (yellow) • E=male: mutant (yellow)

  12. Fruit Fly Body Color

  13. Sex combs: Male Drosophila

  14. Female and Male Fruit Flies

  15. Abdominal segements • 7 segments to female abdominal segments • 5 segments to male abdominal segments • Male body size is smaller than female • But the “last” abdominal segment is larger in male

  16. Experiment 2 3 point test cross Eye color Body color Wing length 2- Experiment: 3 point test cross:

  17. 1. Body Color 2. Wing length 3. Eye Color ♦Wt vs. Mutant Beige (bl+) vs. Black (bl) Long(vg+) vs. Vestigal(vg) Brick red (br+) vs. brown (br) 3 point test cross for:

  18. Crosses: Female Males • Males and female progeny from this F2 cross are your 3 point test cross results! bl+ vg+ Bw+ bl vg bw X bl vg bw bl vg bw

  19. There are all sorts of eyes!

  20. Eyes

  21. You will have only red eyes (wt)

  22. Fruit Fly Wings

  23. Nap TimeTriethylamine

  24. How to anesthetize a fly

  25. How to use the Napper

  26. View the Fruit Fly

  27. You will record class data • After counting the crosses record the data on the class spreadsheet

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