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Biobrick

Biobrick. An adjective, not a noun. http://en.wikipedia.org/wiki/Biobrick. Overview. Short introduction to biobrick parts and plasmids How to make plasmids with biobrick parts How to combine the biobrick parts in one plasmid. B iobrick part in a plasmid.

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Biobrick

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  1. Biobrick An adjective, not a noun http://en.wikipedia.org/wiki/Biobrick

  2. Overview • Short introduction to biobrick parts and plasmids • How to make plasmids with biobrick parts • How to combine the biobrick parts in one plasmid

  3. Biobrick part in a plasmid http://partsregistry.org/Help:An_Introduction_to_BioBricks

  4. The plasmid (backbone) http://partsregistry.org/Help:Plasmid_backbones/Glossary http://partsregistry.org/Help:Plasmids

  5. Construction plasmids http://partsregistry.org/Part:BBa_P1010

  6. Prefix & Suffix Prefix: GAATTCGCGGCCGCTTCTAGAGCTTCCGCGCCGGCGAAGATCTC EcoRIXbaISpeIPstINotI Suffix: TACTAGTAGCGGCCGCTGCAG ATGATCATCGCCGGCGACGTC Prefix cut with XbaI:...GCTT^CTAG AG...CGAA GATC^TC Suffix cut with SpeI:TA^CTAG TAGC...AT GATC^ATCG... SpeI + XbaI ...TA^CTAG AG......AT GATC^TC... http://partsregistry.org/Help:Plasmid_backbones/Glossary http://partsregistry.org/Help:BioBrick_Prefix_and_Suffix

  7. Standard assembly http://partsregistry.org/Help:Standard_Assembly_%28zoom%29

  8. RBS-CDS issues XbaI + SpeI (RBS)TA^CTAG AG(CDS)(RBS)AT GATC^TC(CDS) Scars: Normal:(RBS)TACTAGAG(CDS) Scar of 8 basesWith T:(RBS)TACTAGATG(CDS-ATG) Scar of 6 bases http://partsregistry.org/Assembly:RBS-CDS_issues

  9. Parallel/Rolling assembly http://partsregistry.org/Assembly:Rolling_assembly

  10. Robotic assembly epMotion5075 http://partsregistry.org/Assembly:Robotic_assembly

  11. Questions http://www.firstnews.co.uk/bored/can-you-do-this-word-search-i1919

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