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BioBrick: Aggregate Parts

BioBrick: Aggregate Parts. Coding Region. RBS. Promoter. RBS. Terminator. Reporter Coding Region. Terminator. Reporter. Quad Part Inverter (QPI). QPIs and Reporters. Have. Need. LacI QPI. LacI(ts) QPI. Lambda cI QPI. Lambda cI857 QPI. 434 cI QPI. mCherry. GFP (mut3b). Venus.

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BioBrick: Aggregate Parts

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  1. BioBrick: Aggregate Parts Coding Region RBS Promoter RBS Terminator Reporter Coding Region Terminator Reporter Quad Part Inverter (QPI)

  2. QPIs and Reporters Have Need LacI QPI LacI(ts) QPI Lambda cI QPI Lambda cI857 QPI 434 cI QPI mCherry GFP (mut3b) Venus

  3. Test Constructs Plambda GFP (mut3b) Plambda Lambda cI QPI LacI QPI LacI QPI GFP (mut3b) PLac GFP (mut3b) Lambda cI QPI Plambda GFP (mut3b) Plambda Lambda cI QPI 434 QPI LacI(ts) QPI GFP (mut3b) Plambda GFP (mut3b) LacI(ts) QPI P434 GFP (mut3b) P434 434 QPI 857 QPI 857 QPI GFP (mut3b)

  4. BioBricks Testing

  5. Things We Want to Know • Minimal exposure time to UV to switch state • Minimal concentration of IPTG to switch state • Minimal exposure time to heat to switch state • Amount of time a given state is maintained • Time delay between input and reporting • Effect of varying cell densities • Effect of competing inputs • Does SOS work in stationary phase • Effectiveness of UV laser pointer

  6. Schedule - Week 1

  7. Schedule - Week 2

  8. Schedule - Week 3

  9. Schedule - Week 4

  10. Schedule - Week 5

  11. Schedule - Week 6

  12. Schedule - Week 7+ • Padding

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