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第八章 克隆基因的表达 Expression of Cloned Genes

第八章 克隆基因的表达 Expression of Cloned Genes. Gene Organization. Gene Expression and Regulation Levels. The lac Operon. Structure and expression of the mammalian β-globin gene. Transcription and Translation. Transcription in Prokaryotes. Structure of a typical promoter in E.coli.

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第八章 克隆基因的表达 Expression of Cloned Genes

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  1. 第八章 克隆基因的表达Expression of Cloned Genes

  2. Gene Organization

  3. Gene Expression and Regulation Levels

  4. The lac Operon

  5. Structure and expression of the mammalian β-globin gene

  6. Transcription and Translation

  7. Transcription in Prokaryotes Structure of a typical promoter in E.coli

  8. S-D Recognition Sequences in Prokaryotic mRNAs

  9. Termination of RNA Chains

  10. Expression Vector

  11. Secretion Cloning Vector

  12. Fusional ExpressionVector Factor Xa---Activated form of factor x that participates in both the intrinsic and extrinsic pathways of blood coagulation. It catalyses the conversion of prothrombin to thrombin in conjunction with other cofactors.

  13. Conserved Sequences in Promoters

  14. Distance of Structure Genes and Promoters

  15. Gene expression level and secondary structure of mRNA

  16. Alteration of the Distance between Gene and Promoter

  17. Translation Reading Frame

  18. Construction the Reading Frame

  19. Induced Expression of Gene • Chemical---lac promoter and IPTG IPTG即Isopropyl β-D-1-thiogalactopyranoside，也称Isopropyl β-D-thiogalactoside，中文名为异丙基-β-D-硫代半乳糖苷。分子式为C9H18O5S ，分子量为238.30。 -IPTG, cell growth +IPTG, target gene expression

  20. Induced Expression of Gene • Physical---λPL and temperature sensitive(λcI875 lysogen,32℃,42℃)

  21. Induced Expression of Gene Promoter: λPLλPR Terminator: rrnBT1T2 Supression: 32℃ Gene expression: 42℃

  22. Transcription and Translation in SV40 Virus

  23. SV40 Virus Vector

  24. SV40 Virus Vector and COS Cells

  25. Expression Vectors for Mammalian Cells

  26. Gene Expression in Mammals

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