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Homogenization

Homogenization. Erin Bowering Kevin Gadient Vanessa Hoy CHEE 450 March 27, 2009. Homogenization Process. Mechanical process that breaks down particles or droplets in liquid into smallest size possible (submicron) Fluid subjected to pressure in order to create stable dispersion

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Homogenization

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  1. Homogenization Erin Bowering Kevin Gadient Vanessa Hoy CHEE 450 March 27, 2009

  2. Homogenization Process • Mechanical process that breaks down particles or droplets in liquid into smallest size possible (submicron) • Fluid subjected to pressure in order to create stable dispersion • Flow controlled in homogenizer valve to create ideal conditions for subdividing of particles

  3. Purpose • Objective of homogenization process is to lyse cells into smaller subparticles http://www.sonicmixing.com/homogenizer/

  4. Limitations of SuperPro • “Models” the cell breakage into inclusion bodies • Inconsistencies • Pressure and temperature changes have no effect • Pressure = 1 atm??? • Unreliable source for materials of construction • Difficult to optimize

  5. Homogenizer Design • Continuous 2520 LPH when in use (SuperPro) • Moderately large throughput • Fewer passes at higher pressure chosen. • 2 passes at 1500 bar • Results in 95% cell breakage • Extra Design Considerations • Used linear approximation to determine unit volume of 22.5 cubic meters. • The unit size is large enough to connect to CIP and SIP systems for maintainability.

  6. Cell Disruption Alternatives: Ultrasonic Cell Disruption • Mechanical method that uses inaudible ultrasound to inactivate and disrupt cells in suspension • Cavitation process causes formation of shock waves that break down cell wall • Advantage: highly effective and simple at lab scale • Disadvantage: ineffective at large scale due to high energy requirements and noise safety issues

  7. Cell Disruption Alternatives: Cell Lysis Using Detergents • Non-mechanical method that breaks lipid barrier of cell walls • Advantage: milder alternative to physical disruption of cell walls • Disadvantage: Risk of contamination of product and may be a slower process

  8. Questions?

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