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Microbe-I

Instructor:. Takashi Yamasaki Satoshi Kawaguchi. (yamasaki.takashi@jaxa.jp) (kgucci@jamss.co.jp).

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Microbe-I

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  1. Instructor: Takashi Yamasaki Satoshi Kawaguchi (yamasaki.takashi@jaxa.jp)(kgucci@jamss.co.jp) Notice : This technical data is furnished on the condition that it will be used by and disclosed to the receiving Cooperating agency and its contractors and sub contractors only for the purposes of fulfilling the cooperating agency’s responsibilities under the Space Station Intergovernmental Agreement (IGA) and Memorandum of Understanding (MOU). It shall not be used for any other purpose, nor disclosed or retransferred to any other entity or government without prior written permission of the Japan Aerospace Exploration Agency (JAXA). JEM MICROBE-I 31407 Microbe-I

  2. Objectives After this lesson the trainee will be able to: • Microbe-I Overview - Explain the science objective of the Microbe-I experiment. - List onboard outcomes from performing Microbe-I experiment. - List two sampling methods of Microbe-I experiment. - Explain what crew has to do to Microbial Detection Sheet after sampling to prevent bio contamination. - Explain what crew has to do with BZK Wipe after Microbial Detection Sheet sampling to prevent bio contamination. - Explain what crew must NOT contaminate throughout sampling. - Identify the area on Microbial Detection Sheet Yeast and Mold where crew must NOT touch. - Identify the area on Blue/White Wipes where crew must NOT touch. - Perform Microbe-I operations according to ODFs without instructor’s assistance. • Microbe-I Operations Overview • Key points during Microbe-I operations • Microbe-I Operations

  3. Contents: - Microbe-I Overview - Microbe-I Operations Flow - Key Points during Microbe-I Operations - Appendix Page: 4 5 6 7 Lesson Outline

  4. Microbe-I Overview Microbe-I Science Objective The purpose of this experiment is to monitor microbes in Kibo which may affect the health of crew members. Onboard Outcomes from Microbe-I Experiment • Microbial Detection Sheet Yeast and Mold • White Wipe in White Tube • Blue Wipe in Blue Tube • Photos of Microbial Detection Sheet Yeast and Mold and sampling locations White Wipe and White Tube (for freezing) Blue Wipe and Blue Tube (for refrigeration) Microbial Detection Sheet Yeast and Mold

  5. LEGEND: :Crew operations Microbe-I Operations Flow Microbe-I Operations Flow Photographing Sampling Location Microbial Detection Sheet Sampling Blue/White Wipe Sampling Blue/White Wipe Sampling Microbial Detection Sheet Preparation Blue/White Wipe MELFI Insertion Microbial Detection Sheet Sampling Microbial Detection Sheet Incubation (7 days) Photographing Microbial Detection Sheet Microbial Detection Sheet MELFI Insertion

  6. Key Points during Microbe-I Operations CAUTION: Crew safety may be changed in future. (Final Payload Safety Review Panel has not been held yet.)

  7. Appendix - Diffuser - Acronyms

  8. Diffuser

  9. BZK Wipes MELFI Benzalkonium Chloride Antiseptic Towelette Minus Eighty degree Laboratory Freezer for ISS Acronyms - Benzalkonium chloride antiseptic towelettes are alcohol-free wet wipes used for general antiseptic cleansing.

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