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Peter Bottomley, Daniel Arp, Mark Dolan, and Lewis Semprini , Oregon State University

Aerobic (Co)Metabolism of Chlorinated Ethenes by Microorganisms that Grow on Organic Acids and Alcohols. Peter Bottomley, Daniel Arp, Mark Dolan, and Lewis Semprini , Oregon State University. Induction of BMO activity in response to 50 μ M CAH. Research Objectives:

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Peter Bottomley, Daniel Arp, Mark Dolan, and Lewis Semprini , Oregon State University

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  1. Aerobic (Co)Metabolism of Chlorinated Ethenes by Microorganisms that Grow on Organic Acids and Alcohols Peter Bottomley,Daniel Arp, Mark Dolan, and Lewis Semprini, Oregon State University Induction of BMO activity in response to 50 μM CAH • Research Objectives: • Isolate cultures capable of direct metabolism of cis-dichloroethene and vinyl chloride and characterize their growth kinetics and substrate range. • Conduct biochemical and transformation studies with Pseudomonas butanovora to characterize induction of monooxygenases and provision of reductant for the monooxygenase-catalyzed reaction. • Conduct reactor tests where rates of substrate addition can be controlled, including batch reactor quasi-steady-state tests and chemostat tests, to evaluate catabolite repression. Induction of lacZ activity in response to 50 μM CAH

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