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ProteoRed WG1-WG2 Meeting Pamplona, December 10th 2013

PME10 PROPOSALS. ProteoRed WG1-WG2 Meeting Pamplona, December 10th 2013. MSRT1 SIGMA-ALDRICH. PME10. Objectives : 1) Test Rt standards for SRM methods inter- platform interchangeability 2) Multilaboratory test targeted quantification of Chr16 proteins. Experiment Design :

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ProteoRed WG1-WG2 Meeting Pamplona, December 10th 2013

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  1. PME10PROPOSALS ProteoRed WG1-WG2 Meeting Pamplona, December 10th 2013

  2. MSRT1 SIGMA-ALDRICH

  3. PME10 • Objectives: • 1) Test Rtstandardsfor SRM methods inter-platforminterchangeability • 2) Multilaboratory test targetedquantification of Chr16 proteins • ExperimentDesign: • Sample: total lysateextract of a modelcell line in SpHPP-Chr16 ? • SRM SpHPPvalidatedmethods • Rtstds- Internal / External - Differentgradientlenghts 30-60-90 min • Labeledpeptidestandardsforselectedproteins-> absolutequantitation

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