Metabolic Pathways

2. Metabolic Pathways • Metabolism and Cell Structure • ATP and Energy • Important Coenzymes in Metabolic Pathways • Coenzyme NAD+ • Coenzyme FAD • Coenzyme A

3. Metabolic Pathways

4. Metabolism Metabolism involves: • Catabolic reactions that break down large, complex molecules to provide energy and smaller molecules. • Anabolic reactions that use ATP energy to build larger molecules.

5. Stages of Metabolism Catabolic reactions are organized as stages: • In Stage 1, digestion breaks down large molecules into smaller ones that enter the bloodstream. • In Stage 2, molecules in the cells are broken down to two- and three-carbon compounds. • In Stage 3, compounds are oxidized in the citric acid cycle to provide energy.

6. Stages of Metabolism

7. Cell Structure Metabolic reaction occur in specific sites within cells.

8. Cell Components and Function

9. ATP and Energy • In cells, energy is stored in adenosine triphosphate (ATP).

10. Hydrolysis of ATP • The hydrolysis of ATP to ADP releases 7.3 kcal (31 kJ/mole). ATP ADP + Pi + 7.3 kcal (31 kJ/mole) • The hydrolysis of ADP to AMP releases 7.3 kcal (31 kJ/mole). ADP AMP + Pi + 7.3 kcal (31 kJ/mole)

11. Hydrolysis of ATP to ADP and ADP to AMP

12. ATP and Muscle Contraction • Muscle fibers contains filaments of actin and myosin. • When a nerve impulse increases Ca+2, the filaments slide closer together to contract muscle. • The hydrolysis of ATP in muscle provides the energy for contraction. • As Ca+2 and ATP decrease, the filaments return to the relaxed position.

13. ATP and Muscle Contraction

14. Coenzyme NAD+ • In cells, the oxidation of compounds provides 2H as 2H+ and 2e- that reduce coenzymes. • NAD+(Nicotinamide Adenine Dinucleotide) participates in reactions that produce a carbon-oxygen double bond (C=O). Oxidation O || CH3—CH2—OH CH3—C—H + 2H+ + 2e- Reduction NAD++ 2H++ 2e- NADH + H+

15. Structure of Coenzyme NAD+ • NAD+ (nicotinamide adenine dinucleotide) contains ADP, ribose, and nicotinamide. • NAD+ reduces to NADH when the nicotinamide group accepts H+ and 2e-

16. Coenzyme FAD • FAD participates in reactions that produce a carbon-carbon double bond (C=C). Oxidation —CH2—CH2— —CH=CH— + 2H+ + 2e- Reduction FAD + 2H+ + 2e- FADH2

17. Structure of Coenzyme FAD • FAD(flavin adenine dinucleotide) contains ADP and riboflavin (vitamin B2) • FADreduces to FADH2 when flavin accepts 2H+ and 2e-

18. Coenzyme A • CoA activates acyl groups such as the two-carbon acetyl group for transfer. O O || || CH3—C— + HS—CoA CH3—C—S—CoA