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International Conference on Livestock Nutrition August, 11 - 12, 2015 Frankfurt , Germany

This study aims to determine the type of mold (Aspergillus niger, Eupenicillium javanicum, Sclerotium rolfsii), substrate composition, and fermentation time that can optimize cellulase, mannanase, and protease enzyme activities in Palm Kernel Cake (PKC) for poultry feed.

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International Conference on Livestock Nutrition August, 11 - 12, 2015 Frankfurt , Germany

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  1. International Conference on Livestock Nutrition August, 11 - 12,2015 Frankfurt , Germany THE EFFECT OF MOLD, FERMENTATION TIME AND SUBSTRATE COMPOSITION OF PALM KERNEL CAKE ON CELULOSE AND MANANNASE ENZYME ACTIVITIES Prof. Dr.Ir. MIRNAWATI, MS Dr. Ir. ADE DJULARDI, MS Dr. Ir. GITA CIPTAAN, MP FACULTY OF ANIMAL SCIENCE ANDALAS UNIVERSITY, PADANG WEST SUMATERA, INDONESIA

  2. BACKGROUND FEED COST of POULTRY is HIGH PALM KERNEL CAKE (PKC) • POTENTIAL • PALM OIL PRODUCTION 23 TONS • INCREASE EVERY YEAR (18%) • HIGH PROTEIN (20,04 %) • AS A FEED STUFF FOR LIVESTOCK CONSTRAINTSFor POULTRY - HIGH FIBER (21.75%) - LOW DIGESTIBILITY (53%) - HIGH MANAN CONTENT( 50% from Fiber) - Usage 5 - 10 % IN POULTRY RATION - LOW AMINO ACID BIOTECHNOLOGY FERMENTATION With M.O. CELULOLITICand MANANOLITIC • PROFIT • DECREASE CRUDE FIBER(11.37%) • INCREASED CRUDE PROTEIN PK (26.27%, WEAKNESS USAGE IN LIMITED RATION FOR POULTRY (17%), BECAUSE IT CONTAIN NUCLEIC ACID

  3. PKC (CRUDE FIBER AND MANAN ARE HIGH) CELULOLITIC AND MANNANOLITIC OF MOLD Aspergilus niger Eupenicilium javanicum Sclerotium rolfsii To produce cellulose and mananaseenzymes Substrat Composition of PKC Fermentation Time Qualified enzyme production Using Enzyme will Increase PKC in poultry rations

  4. THE AIM OF EXPERIMENT The aim of this study was to determine the type of mold sellulolitic and mananolitic (Aspergillusniger, Eupenicilliumjavanicum and Sclerotiumrolfsii), substrate composition of PKC and the optimum fermentation time on the activities of cellulase , mannanase and protease enzyme so as to increase the use of PKC in poultry rations.

  5. THE EXPERIMENTAL METHOD • The experiment used a Completely Randomize Design (CRD) in a 3x3x3factorial with3replications • The First Factor: various types of mold (1.Aspergilus niger, 2. Eupenicillium javanicum, 3.Sclerotium rolfsii) • The Second Factor: Substrate composition (1. 5% PKC, 2. 8% PKC, 3. 11% PKC) • The ThirdFactor: Fermentation Time ( 36, 48, 60 hours) • Parameters: Enzyme activity of cellulase, manannase and proteasefrom fermented Palm Kernel Cake.

  6. RESULT OF EXPERIMENT

  7. CONCLUSION The fermentation of PKC withSclerotium rolfsii and substrate composition 11 % of PKC and Fermentation time 60 hours give enzyme activity of cellulose, mananase and protease better thanAspergilus niger and Eupenicillium javanicum. Theactivity of cellulase, mannanase, and protease are as follows: 20.74 U/ml, 9.81%, and 16.05 U/ml

  8. THANK YOU

  9. 6. ATTACHMENT A. niger

  10. 6. ATTACHMENT E. javanicum

  11. 6. ATTACHMENT S. Rolfsii

  12. 6. ATTACHMENT Sterilization and autoclave

  13. 6. ATTACHMENT Inoculation of Mold

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