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Bacterial DNA Transformation: Methods and Mechanisms Explored

Discover the diverse methods of bacterial DNA transformation, including natural and induced competence, electroporation, transduction, and conjugation. Learn about key bacteria involved and the process of gene transfer.

alfonso-roberts
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Bacterial DNA Transformation: Methods and Mechanisms Explored

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  1. Transformation • A DNA fragments/plasmids of donor bacteria • Taken up & incorporated into the genome of recipient cell • Natural competence Streptococcus pneumoniae, Haemophilus influenzae, Neisseria gonorrhoea • Induced competence Cold CaCl2 & heat shock: E. coli Protoplast: Bacillus, Streptomyces Electroporation: a brief pulse of high-voltage electricity

  2. Transduction Generalized transduction Specialised transduction Transfer of DNA by Bacteriophage via Lytic & Lysogenic cycle

  3. Conjugation Discovered 1946 Lederberg & Tatum Donor: • Containing conjugative plasmid (F+) Recipient: • Bacterial receiving the plasmid (F-) High-frequency recombination (Hfr)

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