1. Large-Scale Protein Production INQUIRY Large-scale recombinant protein production is becoming increasingly important for applications in the field of proteomics, structural biology and protein therapeutics. When large quantities of protein (from mg to gamounts) needs to be produced from either bacterial (e.g. Escherichia coli) or eukaryotic cell-based culture (e.g. yeast, insect and mammalian cell), cells must be cultured in large volume by the implementation of fermentation techniques. Fermentation can improve efficiency by increasing cell density and provide easy-to-handle large culture over 4 liters. Depending on the high cell density as well as fermentation strategy, we can optimize cell culture conditions to increase cell mass and finally improve recombinant protein yield. Profacgen is fully equipped to meet this production demand for large quantities of pure and active protein. Our versatile protein expression platform is equipped with a comprehensive series of bioreactors (from fermenters to wave mixers), which can accommodate protein production projects at different scales, ranging from milligrams to hundreds of grams. Capabilities and Specifications of Different Expression Systems Expression System Expression System Capabilities Capabilities Features Features Bacteria Bacteria Fermentation culture up to 2000 L i. Homogeneous recombinant protein production with purity over 98% for crystallization. ii. Protein yield up to several grams. Yeast Yeast Fermentation culture up to 2000 L i. Homogeneous recombinant protein production with purity over 98% for crystallization. ii. Protein yield up to grams. Insect/Baculovirus Insect/Baculovirus Culture up to 2000 L i. Homogeneous recombinant protein production with purity over 98% for crystallization. ii. High titer recombinant Baculovirus stock. iii. Protein yield up to hundreds of milligrams. Mammalian Cells Mammalian Cells Stable cell line culture up to 500 L i. Protein yield up to milligrams. ii. Allow transient expression for the production of

2. Expression System Expression System Capabilities Capabilities Features Features up to 10 mg protein. From Bench-Top Fermenter to Bioprocess Scale-Up Facility Improvements for Upstream and Downstream Technologies Creation of Highly Productive Cell Creation of Highly Productive Cell Lines Lines Modified gene vectors combined with optimized transfection and Modified gene vectors combined with optimized transfection and screening methods screening methods Engineered cell lines also improve protein characteristics Engineered cell lines also improve protein characteristics Select highly productive clones Select highly productive clones Fermentation Optimization Fermentation Optimization Improve media and feeding strategies Improve media and feeding strategies Optimize growth and physicochemical conditions Optimize growth and physicochemical conditions Profacgen provides you with an ultimate solution for large-scale protein production. Our service can be tailored according to your specific requirements. Our expertise guarantees high protein quality, short turnaround time, and best price in the market! For more information or project quotations, you are welcome to contact us at 1-631-559-9269, or send an email to .

3. INQUIRY