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Genesis 1:26

Genesis 1:26 26 And God said, Let us make man in our image, after our likeness: and let them have dominion over the fish of the sea, and over the fowl of the air, and over the cattle, and over all the earth, and over every creeping thing that creepeth upon the earth. DNA Technology.

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Genesis 1:26

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  1. Genesis 1:26 26 And God said, Let us make man in our image, after our likeness: and let them have dominion over the fish of the sea, and over the fowl of the air, and over the cattle, and over all the earth, and over every creeping thing that creepeth upon the earth.

  2. DNA Technology Timothy G. Standish, Ph. D.

  3. What is DNA Technology? • Technology = The application of science in industry and/or commerce. • Because DNA is the genetic material, manipulation of DNA (genetic engineering) allows the modification of organisms in such a way that they are more valuable than they were in their natural state.

  4. Products of DNA Technology • Recombinant organisms - Some examples: • Herbicide resistant soy beans • Super tomatoes • Natural products produced by recombinant organisms: • Humulin - Human insulin produced in cell culture • Tissue Plasminogen Activator (TPA) • Engineered products produced by recombinant organisms: • Amplitaque - A modified DNA polymerase • Information: • Genetic testing for identification of diseases, individuals etc... • DNA sequencing

  5. A Short History • 1869 - Miescher isolated DNA for the first time • 1944 - Avery et al. provided evidence that DNA is the genetic material • 1953 - Watson and Crick described the double helix structure of DNA • 1957 - Kornberg discovered DNA polymerase • 1961 - Marmer and Doty discovered DNA renaturation • 1962 - Arber, Nathans and Smith discovered restriction endonucleases • 1966 - Nirenberg, Ochoa, and Khorana figured out the genetic code.

  6. A Short History Cont. • 1967 - Geller discovered DNA ligase • 1972-73 - Boyer, Cohen and Berg develop DNA cloning techniques • 1975 - Southern developed gel-transfer hybridization • 1975-77 - Sanger and Barrel and Maxam and Gilbert developed DNA sequencing methods • 1981-82 - Palmiter and Brinster produced transgenic mice, Spradling and Rubin produced transgenic fruit flies • 1985 - Mullis and colleagues invented the Polymerase Chain Reaction (PCR)

  7. Extract DNA Fragment DNA Insert into vector DNA Library in bacteria Screen library and grow up bacteria with clone of interest Strategy

  8. The End

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