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Team Division into Dry and Wet Lab for DNA Synthesis and Protocol Development

This week, our team has split into Dry Lab and Wet Lab activities. The Wet Lab team, consisting of Erika, James, Prudence, Chris, Clinton, Tom, Yanis, and Krupa, is focused on two key tasks: DNA synthesis and the development of protocols and reagents. Key components include promoters, RBS, genes, and terminators. We aim to test culturing and transformation using an integration vector, and upon success, will linearize the vector for linear integration. Additionally, microscope training is scheduled for Thursday, alongside protocol refinement for testing constructs.

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Team Division into Dry and Wet Lab for DNA Synthesis and Protocol Development

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  1. Week 6 Team has split into Dry Lab and Wet Lab Dry Lab: Wet Lab: • Erika, - James, • Prudence, -Chris, • Clinton, -Tom, • Yanis, -Krupa,

  2. Wet Lab Two key tasks: • DNA synthesis • Protocols and Reagents

  3. Promoter and RBS Genes Terminators DNA Synthesis

  4. Promoter and RBS Promoters Inducible 1.Phyper-spank 2.pXyl Constitutive 1.p43 2.pVeg Sigma B 1.pctc 2.pgsiB RBS =12 combinations 1.gsiB 2.spoVG

  5. Genes Genes Light+Motility 1.YtvA 2.EspE Signal Peptide 1.SacB 2.LipA Biomaterials 1.Elastin 2.EAK 16 Registry 1.Antibiotics 2.GFPmut3b =4 Combinations

  6. Terminators Insertion Sequence Terminators Registry 1.Double Terminator Insertion Sequence 1.PyrD 2.AmyE

  7. Protocols • Cloning, • Culturing, • Transformation, • Testing,

  8. Cloning

  9. Culturing and Transformation This week we are planning to test culturing and transformation using integration vector. If successful will linearise vector and test for linear integration

  10. Testing • Have defined testing Constructs. • This week need to work out testing protocols, • Microscope training on Thursday!

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